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Year Month   
2017/9  Seminars
Seminar Title An adaptable phospholipid membrane mimetic system for in vitro studies of membrane proteins
Time Y/M/D 2017/9/7 11:00 ~ 12:00
Venue IBC R114
Speaker Mr. Chih-Ta Henry Chien
Current Position 英國劍橋大學生化所博士班學生
Host Dr. Shang-Te Danny Hsu
Contact Nancy Liu
TEL 02-27855596#1165
E-mail liukchun@gate.sinica.edu.tw
Abstract Around 30% of all genes encode integral membrane proteins (MP), which have a range of important biological functions leading to their involvement in many diseases. Nevertheless, characterisation of their structure and dynamics remains heavily underrepresented. The biggest challenge for in vitro studies of MPs is finding a suitable mimic of the native lipid membrane bilayer, which are sufficiently stabilising for structural studies, but which maintain the MP’s biological function and interactions with ligands and other binding partners. Based on the saposin-A (SapA) scaffold protein we demonstrate the suitability of a size-adaptable phospholipid membrane-mimetic system for solution NMR studies of MPs under close-to-native conditions. The Salipro nanoparticle size can be tuned over a wide pH range by adjusting the saposin-to-lipid stoichiometry enabling maintenance of sufficiently high amounts of phospholipid in the Salipro nanoparticle to mimic a realistic membrane environment while controlling the overall size to enable solution NMR for a range of membrane proteins. Three representative membrane proteins including one G-protein-coupled receptor (GPCR) were successfully incorporated into SapA-dimyristoyl-phosphatidylcholine (DMPC) nanoparticles and studied by solution NMR spectroscopy.-Mr. Chih-Ta Henry Chien
 

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