Home Site Map Chinese
Dr. Steven  Lin
Assistant Research Fellow
Room 503, Institute of Biological Chemistry, Academia Sinica
128, Academia Road Sec. 2, Nankang, Taipei 115, Taiwan
TEL: +886-2-27855696 ext. 5030
FAX: +886-2-27889759
stevenlin@gate.sinica.edu.tw

The CRISPR/Cas9 system is a robust genome editing tool that has revolutionized genetic studies in human cells, animals and plants. The core of technology is the enzyme Cas9, an RNA-guided DNA endonuclease that induces a double-strand break at the guide RNA-specified chromosomal locus of interest. My research focuses on developing methods to enable robust, precise and safe genome manipulations in human genome. Current projects include:

1. Understanding DNA repair mechanism to enable efficient Cas9-mediated homologous recombination.

2. Discovering and characterizing orthogonal CRISPR/Cas systems.

3. Expanding Cas9 ribonucleoprotein nucleofection for transcription and epigenetic regulations.

2006 - 2012 Ph.D., Microbiology, University of Illinois at Urbana-Champaign, USA
2004 - 2006 M.S., Biochemistry, The Ohio State University, USA
1996 - 2001 B.S., Biology, University of British Columbia, Canada

2016,01 - present Assistant Research Fellow, Institute of Biological Chemistry, Academia Sinica, Taiwan
2012 - 2015 Postdoctoral fellow, Department of Molecular and Cell Biology, University of California, Berkeley, USA
2004 - 2004 Research assistant, Department of Biochemistry, The Ohio State University, USA
2001 - 2003 Research assistant, Institute of Chemistry, Academia Sinica, Taiwan

    Publications List
Generation of knock-in primary human T cells using Cas9 ribonucleoproteins.
Schumann K, Lin S, Boyer E, Simeonov DR, Subramaniam M, Gate RE, Haliburton GE, Ye CJ, Bluestone JA, Doudna JA & Marson A Proc. Natl. Acad. Sci. USA (2015)
Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery.
Lin S, Staahl B & Doudna JA eLIFE (2014)
Structures of Cas9 endonucleases reveal RNA-mediated conformational activation.
Jinek M, Jiang F, Taylor DW, Sternberg SH, Kaya E, Ma E, Anders C, Hauer M, Zhou K, Lin S, Kaplan M, Iavarone AT, Charpentier E, Nogales E & Doudna JA Science (2014)
High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity.
Pattanayak V, Lin S, Guilinger JP, Ma E, Doudna JA & Liu DR Nat. Biotechnol. (2013)
Precise and Heritable Genome Editing in Evolutionarily Diverse Nematodes Using TALENs and CRISPR/Cas9 to Engineer Insertions and Deletions.
Lo TW, Pickle CS, Lin S, Ralston EJ, Gurling M, Schartner CM, Bian Q, Doudna JA & Meyer BJ Genetics (2013)
RNA-programmed genome editing in human cells.
Jinek M, East A, Cheng A, Lin S, Ma E & Doudna JA eLIFE (2013)
Structure of the enzyme-ACP substrate gatekeeper complex required for biotin synthesis.
Agarwal V, Lin S, Nair S & Cronan JE Proc. Natl. Acad. Sci. USA (2012)
The BioC O-methyltransferase catalyzes methyl esterification of malonyl-acyl carrier protein, an essential step in biotin synthesis.
Lin S & Cronan JE J. Biol. Chem. (2012)
Synthesis of the α, ω-dicarboxylic acid precursor of biotin by the canonical fatty acid biosynthetic pathway.
Cronan JE & Lin S Curr. Opin. Chem. Biol. (2011)
Biotin synthesis begins by hijacking the fatty acid synthetic pathway.
Lin S, Hanson RE & Cronan JE Nat. Chem. Biol. (2010)

Updated 2017.03.08

Browser & IE Recommendations: 1024*768 / IE 8.0、Firefox 10.0

128, Academia Road Sec. 2, Nankang, Taipei 115, Taiwan Tel : 886-2-27855696 Fax : 886-2-27889759

Copyright © 2013.01 IBC, Academia Sinica. All rights reserved.    Terms of use