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Dr. Kay-Hooi Khoo
Distinguished Research Fellow & Acting Director
Room 603, Institute of Biological Chemistry, Academia Sinica
128, Academia Road Sec. 2, Nankang, Taipei 115, Taiwan
TEL: +886-2-2785-5696 ext. 6030
FAX: +886-2-2788-9759
kkhoo@gate.sinica.edu.tw
Kay-Hooi Khoo's Website


Capitalizing on recent rapid advances in mass spectrometry instrumentation to deliver the much needed improved sensitivity, selectivity and throughput in protein modification analysis, proteomics, glycomics and glycoproteomics, without compromising precision. The current focus areas and enabling MS-based techniques to be developed include:

• high throughput and high sensitivity LC-MS/MS-based mapping and sequencing of complex N- and O-glycans, towards a global and targeted glycomics of sufficient breadth and depth;

• functional sulfoglycomics, towards unraveling myriad sulfated glycotopes implicated in mediating cell-cell interactions, particularly those of human and mouse myeloid and lymphoid cells and tissues;

• glycopeptide sequencing/identification MS data acquisition and analysis workflows, towards efficient localization of biologically implicated and/or structurally interesting glycotopes onto their protein carriers;

• quantitative determination of site-specific Cys modifications and the dynamics of their inter-conversion, particularly those of receptor protein tyrosine phosphatase

1989 - 1992 Ph.D. , Biochemistry, Imperial College, University of London.
1986 - 1989 B.S. , Biochemistry , Imperial College, University of London.

2017,08 - present Acting Director, Institute of Biological Chemistry, Academia Sinica
2012,07 - present Distinguished Research Fellow, Institute of Biological Chemistry, Academia Sinica
2012,07 - 2017,08 Associate Director, Institute of Biological Chemistry, Academia Sinica
2008,03 - 2012,07 Research Fellow, Institute of Biological Chemistry, Academia Sinica
1996 - 2008,03 Associate Research Fellow , Institute of Biological Chemistry, Academia Sinica
1994 - 1996 Postdoctoral Fellow, Colorado State University,
1992 - 1994 Postdoctoral Fellow , Imperial College, University of London.

    Publications List
Concerted mass spectrometry-based glycomic approach for precision mapping of sulfo sialylated N-glycans on human peripheral blood mononuclear cells and lymphocytes.
Chen JY, Huang HH, Yu SY, Wu SJ, Kannagi R, and Khoo KH* Glycobiology (2018)
Adapting Data-Independent Acquisition for Mass Spectrometry-Based Protein Site-Specific N-Glycosylation Analysis.
Pan KT, Chen CC, Urlaub H, and Khoo KH* Anal Chem (2017)
Advancing a high throughput glycotope-centric glycomics workflow based on nanoLC-MS2-product dependent-MS3 analysis of permethylated glycans.
Hsiao CT, Wang PW, Chang HC, Chen YY, Wang SH, Chern Y, and Khoo KH* Mol Cell Proteomics (2017)
Direct Mapping of Additional Modifications on Phosphorylated O-glycans of alpha-Dystroglycan by Mass Spectrometry Analysis in Conjunction with Knocking Out of Causative Genes for Dystroglycanopathy.
Yagi H, Kuo CW, Obayashi T, Ninagawa S, Khoo KH*, and Kato K* Mol Cell Proteomics (2016)
Efficient Mapping of Sulfated Glycotopes by Negative Ion Mode nanoLC-MS/MS-Based Sulfoglycomic Analysis of Permethylated Glycans.
Cheng CW, Chou CC, Hsieh HW, Tu Z, Lin CH, Nycholat C, Fukuda M, and Khoo KH Anal Chem (2015)
Unmasking of CD22 Co-receptor on Germinal Center B-cells Occurs by Alternative Mechanisms in Mouse and Man.
Macauley MS, Kawasaki N, Peng W, Wang SH, He Y, Arlian BM, McBride R, Kannagi R, Khoo KH, and Paulson JC J Biol Chem (2015)
From mass spectrometry-based glycosylation analysis to glycomics and glycoproteomics.
Khoo KH Adv Neurobiol (2014)
Mass spectrometry-based quantitative proteomics for dissecting multiplexed redox cysteine modifications in nitric oxide-protected cardiomyocyte under hypoxia.
Pan KT, Chen YY, Pu TH, Chao YS, Yang CY, Bomgarden RD, Rogers JC, Meng TC, and Khoo KH Antioxid Redox Signal (2014)
Novel LC-MS(2) Product Dependent Parallel Data Acquisition Function and Data Analysis Workflow for Sequencing and Identification of Intact Glycopeptides.
Wu SW, Pu TH, Viner R, and Khoo KH Anal Chem (2014)
Galactose 6-O-sulfotransferases are not required for the generation of Siglec-F ligands in leukocytes or lung tissue.
Patnode ML, Cheng CW, Chou CC, Singer MS, Elin MS, Uchimura K, Crocker PR, Khoo KH, and Rosen SD J Biol Chem (2013)

Updated 2017.03.08

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