Curriculum Vitae

Ming-Daw Tsai, Ph.D.

Institute of Biological Chemistry,

Academia Sinica, Taipei, Taiwan
 
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Personal

Address: 128 Academia Road, Section 2, Taipei 115, Taiwan
Phone: +886-2-2785-5696 ext 1013
e-mail: mdtsai@gate.sinica.edu.tw
Homepage: http://www.bc.sinica.edu.tw/MDTsai/
 
Education

1968-1972, B.S. in Chemistry, National Taiwan University, Taipei
(1972-1974 in compulsory military service)
1974-1978, Ph.D. in Biochemistry & Medicinal Chemistry, Purdue Univ. (Heinz Floss)
 
 
Positions Held

2008 Aug - present Distinguished Research Fellow, Institute of Biological Chemistry, Academia Sinica
2008 Aug - 2014 Aug Director, Institute of Biological Chemistry, Academia Sinica
2007 Aug - present Professor, Institute of Biochemical Sciences, National Taiwan Univ.
2007 Feb - present Director, CBMB Program, Taiwan International Graduate Program
2006 Oct - 2008 Aug Acting Director, Institute of Biological Chemistry, Academia Sinica
2005 Jan - 2008 Aug Director of Functional Genomics, GRC
2004 Jan - 2010 Dec Director, National Core Facilities Office, NRPGM
2003 Nov - 2008 Aug Distinguished Research Fellow, Genomics Research Center, Academia Sinica, Taiwan
2003 Oct – 2007 June Kimberly Professor of Chemistry, Ohio State University
1993 Oct - 2007 June Director, Office of Research Campus Chemical Instrument Ctr, OSU
1992 Jan - 2007 June Professor, Department of Biochemistry, OSU
1990 Oct - 2007 June Professor, Department of Chemistry, OSU
1995 Sept - 2003 July Director, Chemistry/Biology Interface Training Program, OSU
1986 Oct - 1990 Sept Associate Professor of Chemistry, Ohio State University
1989 Aug - 1990 June Visiting Professor, Dept. of Biochemistry, University of Wisconsin, Madison (with John Markley)
1986 Mar - 1986 Sept Visiting Professor, University of Lund, Sweden (with Sture Forsen)
1981 July - 1986 Sept Assistant Professor of Chemistry, Ohio State University
1980 July - 1981 July Assistant Prof of Chemistry, Rutgers Univ. (Newark)
1980 July - 1980 Dec Research Associate, University of Illinois (with Eric Oldfield)
1979 July - 1980 Dec Visiting Assistant Professor, Medicinal Chemistry, Purdue Univ.
1978 Jan - 1979 June Postdoctoral Associate, Purdue University (with H. G. Floss)
 
Research Interest

Mechanism of Action of Enzymes Involving Biophosphates
Mechanism of DNA polymerases involved in DNA repair
Structure and Function of FHA domains in DNA damage signaling
Chemical and structural biology
Biological NMR
 
Honors/Awards

1. Glenn L. Jenkins Award for Excellence in Research, Purdue Univ., 1977.
2. Alfred P. Sloan Fellow, 1983-1985
3. Faculty Research Award, Ohio State Chapter of Sigma Xi, 1985.
4. Camille and Henry Dreyfus Teacher-Scholar, 1985-1990.
5. Distinguished Scholar Award, Ohio State University, 1992.
6. Elected Fellow, American Association for the Advancement of Science (AAAS), 1992.
7. Committee on Institutional Cooperation (CIC) Academic Leadership Program Fellow, 2002-2003.
8. Kimberly Professor of Chemistry, Ohio State University, 2003-2007.
9. Guest Professor, Tsinghua University, PRC, 2003-2006.
10. Distinguished Alumnus Award, Purdue University School of Pharmacy, 2008
11. Academician, Academia Sinica, elected 2012
12. Fellow, The World Academy of Science (TWAS), elected 2014
   
Special Professional Services

1. Symposium Organizer, "Quadrupolar NMR in Biophysical Chemistry", ACS 18th Central Regional Meeting, 
Bowling Green, June 4, 1986.
2. Co-Chairperson, 8th Midwest Enzyme Chemistry Conference, October, 1988.
3. NIH Physical Biochemistry Study Section Member, 1988-92.
4. Co-chair, Gordon Conference on Enzymes, July 1993.
5. Board Member, Chinese American Chemical Society, 1993-96.
6. Co-founder, Ohio NMR Consortium, 1995.
7. Nominating Committee, American Chemical Society Division of Biological Chemistry, 1997.
8. NIH Training Grant Study Section, 1997-01.
9. Symposium Organizer, “NMR of Biological Macromolecules”. 31st Central Regional Meeting of the American Chemical Society, June 21-23, 1999.
10. Advisory Board, Institute of Chemistry, Academia Sinica, Taiwan, 1991-present.
11. President, Chinese American Chemical Society, 2003.
12. Organizer, Frontiers of Bioorganic and Natural Product Chemistry Symposium, Seattle, August 26-29, 2004.
13. Board of Consulting Editors, Bioorganic & Medicinal Chemistry, 1998-2006.
14. Board of Consulting Editors, Bioorganic & Medicinal Chemistry Letters, 1998-2006.
15. Theme Editor, Current Opinions in Chemical Biology, Volume 7, April 2003.
16. Cancer PPG Special Study Section, National Cancer Institute, NIH, October 2-3, 2007.
17. Program Chair, International Society for Magnetic Resonance Conference (ISMAR), Kenting, Taiwan, Oct 14-19, 2007
18. President, Taiwan Magnetic Resonance Society, 2007 - 2010
19. Editorial Advisory Board for Biochemistry, January 2009 – present
20. International Liason for the Symposium of SCBA, 2009.
21. President, Taiwan Society of Biochemistry and Molecular Biology, 2009-2012.
22. Associate Editor, Biochemistry, 2010 – present.
23. President, Taiwan Biophysical Society, 2013 – present.
24. STRENDA (Standards in Reporting Enzyme Data) Commission, Beilstein Institute of Germany, 2014-present
25. Visiting Committee for School of Life Sciences, Chinese University of Hong Kong, July 2014.
   
Mentoring of Graduate Students and Postdoctors
I have trained 55 Ph. D. graduates and 16 master graduates, and 30 postdoctors. A number of former students and former postdoctors have taken faculty positions in academic institutions in the U. S. and Taiwan, as listed below:
1. Yund-Jung (John) Shyy, Ph. D. 1987 (Chemistry), Professor, Department of Bioengineering, University of California at San Diego
2. Charles R. Sanders, Ph. D. 1988 (Chemistry), Professor, Dept. of Biochemistry and Biophysics, Vanderbilt University School of Medicine.
3. Tsung-Chung (Alan) Tsai, postdoctor 1982-85, Professor (retired), Tunghai Univ, Taiwan.
4. William M. Loffredo, Ph. D. 1988 (Chemistry), Professor, Dept. of Chemistry, East Strasburg University, Pennsylvania.
5. Gialih (Hoffman) Lin, Ph. D. 1989 (Chemistry), Professor of Chemistry, Chung-Hsing U., Taiwan.
6.  Joseph P. Noel, Ph. D. in 1990 (Chemistry), Professor, SALK Institute and Dept. of Chemistry, University of California at San Diego.
7. Honggao Yan, Ph. D. 1991 (OSBP), Professor, Dept. of Biochemistry, Michigan State University.
8. Karol Bruzik, former postdoctor, Professor, Dept. of Medicinal Chemistry, University of Illinois at Chicago.
9. Cynthia M. Dupureur (Chemistry), Ph. D. 1992, Associate Professor, Dept. of Chemistry and Biochemistry, University of Missouri, St Louis.
10. Robert Hondal, Ph. D. 1997 (Chemistry), Assistant Professor, Department of Biochemistry, University of Vermont.
11. In-Ja L. Byeon, Senior NMR Research Scientist, Department of Structural Biology, University of Pittsburgh School of Medicine.
12. Karen Ericson, Ph. D. 1998 (Chemistry), Assistant Professor at Indiana University-Purdue University Fort Wayne.
13. Thomas Selby, Ph. D. 1999 (Chemistry), Assistant Professor, Central Florida State University.
14. Junan Li, Ph. D. 2000 (Biochemistry), Postdoctor 2002-2005, Assistant Professor, College of Public Health, OSU, 2007.
15. Paul O’Maille, Ph. D. 2001, Assistant Professor, Department of Metabolic Biology, John Innes Centre (JIC) Plant Natural Products and Health, Institute of Food Research (IFR) Colney Lane, Norwich NR4 7UH, UK.
16. Kuo-Hsiang Joseph Tang, Assistant Professor, Department of Chemistry and Biochemistry, Clarkson University, Potsdam, New York.
17. Yi Guo, Ph. D. 2009 (OSBP), Assistant Professor (2012 – present), Department of Health Outcomes and Policy, College of Medicine, University of Florida, Gainesville, FL.
18. Jinwoo Ahn, Assistant Professor (2012 – present), Dept of Structural Biology, University of Pittsburgh, PA.
   
Professional Membership

American Chemical Society (USA)
American Society of Biochemistry and Molecular Biology (USA)
American Association for the Advancement of Science (Elected Fellow in 1992) (USA)
Taiwan Biophysical Society
Taiwan Magnetic Resonance Society
Taiwan Society of Biochemistry and Molecular Biology
 
Training Grants (as Principal Investigator/Program Director)

1. "Chemistry/Biology Interface Training Grant", NIH, T32 GM 08512, 7/1/96-6/30/01, annual direct cost ca. $300,000; renewed for 7/1/01-6/30/06. (Dr. Dehua Pei took over the directorship and the PI starting 7/1/03).
   
Equipment Grants (as Principal Investigator only; co-PI of many other proposals.)

1. "Purchase of a 600 MHz NMR Spectrometer", $200,000, NIH, RR 08299, 8/15/93-8/14/94.
2. "Purchase of a 600 MHz NMR Spectrometer", $200,000, NSF, BIR-9221639, 6/1/93-5/31/95.
3. "500 MHz Console Upgrade", $258,000, NSF, 5/96.
4. "800 MHz NMR", $1,870,000, Ohio Board of Regents, 4/96.
5. "Ohio NMR Consortium", $2,000,000, Ohio Board of Regents (the funding is distributed among several universities in Ohio; Tsai serve as PI of the Consortium proposal), June 2002.
   
Past Research Grants(as Principal Investigator)

1. "Stereochemistry of Enzyme Reactions Involving a Proprochiral Phosphorus Center ". (NSF) 8/1/79 - 7/31/82, Total cost: $108,000.
2. "17O and 31P NMR of Biophosphates". (NIH) GM 29041, 8/1/82 - 11/30/85, Direct cost: $182,545.
3. Research Award, A. P. Sloan Foundation, 9/15/83 - 9/14/85, Total cost: $25,000.
4. Dreyfus Teacher-Scholar Award, 12/1/85 - 11/30/90, Direct Cost: $47,000, Total cost: $50,000.
5. "Productive Versus Nonproductive Binding of Kinases". (NSF) DMB-8603553, 9/86 - 8/89, Total Cost: $232,133.
6. "Mechanism of Adenylate Kinase". (NSF) DMB 89-04727, 3/90-2/93, Tootal Cost: $285,000.
7. "The Role of Aspartate in the Catalytic Diad". NIH, F32 GM15973, 12/93 - 11/96, direct cost $72,900. (Postdoctoral Fellowship to Brian Werneburg).
8. "Stereochemistry of Enzyme Reactions at Phosphorus", NIH, GM30327, 7/79 – 11/93, final year direct cost $112,585.
9. "Mechanism of Inositide-Related Enzymes", NIH, GM30327, 12/93 - 11/97, final year direct cost $137,578. This was continuation of GM30327 with a change of title.
10. "Mechanism of p19ARF – Bridging Two Major Tumor Suppression Pathways", PI, a grant from American Cancer Society through Comprehensive Cancer Center, $35,000 from 7/1/98 to 6/30/99.
11. "Mechanism of Adenylate Kinase". NIH, GM43268, 8/92 – 7/96, final year (year 04) direct cost $155,357.
12. "Mechanism of Phospholipase A2". NIH, GM41788, 4/89 – 3/01, final year (year 12) direct cost $153,514.
13. "Structure Function of FHA Domain in Signaling and Cancer". NIH, CA87031, 3/1/01-2/28/05 (four years), final year direct cost $157,000.
14. "Conformational Changes in Phospholipase C", NIH, GM57568, 12/98-12/08. This is continuation of GM30327. K. Bruzik became the PI of this grant. My share is ca. $50K/year direct cost.
15. "Mechanism of DNA Polymerases". NIH GM43268, 12/97 – 6/06, final year (year 12) direct cost $222,300.
16. "Functional Genomics Approach to the Virulence of Klebsiella pneumonia". AS, 94F008-1, PI, 1/05-12/07, current year cost $NT3,000,000.
17. "Functional and Genomic Studies of Capsular synthesis Region of Tissue Invasive Klebsiella pneumoniae". NSC, 95-3112-B-001 -021-, PI, 08/06-07/07, current year total cost $NT2,500,000.
18. "Structure-Function Relationship of Tumor Suppressors". NIH, CA69472, PI, 6/00-6/09, final year total cost $292,000.
19. "Mechanism of Catalysis by the RNA Polymerase of Avian Flu Virus". NSC, 95-2745-B-001 -004-, PI, 08/06-07/09, total cost $NT7,500,000.
20. "Enzymes in the biosynthesis of the capsular polysaccharide of Klebsiella pneumoniae". NSC, 98-3112-B-001 -003-, PI, 05/07-04/10, current year cost $NT5,950,000, total cost $NT18,331,000. Extended to 04/11.
21. "Protein-Phosphoprotein Interaction in Cancer-related Signaling". NHRI, EX95-9508NI, PI, 1/06-12/10, current year total cost $NT2,355,000.
   
Active Research Grants

2011 Academia Sinica Investigator Award, Academia Sinica
計畫編號: 100-深耕計畫-11
補助期限: 2011-1-1~2015-12-31
Research Theme:
Mechanism of biophosphates: from DNA repair polymerases to DNA damage signaling
生物磷酸鹽之反應機制:從DNA修復聚合酶到DNA損害之訊息傳遞
Annual support: 2,000,000 NTD
NHRI
計畫編號: NHRI-EX100-10002NI
補助期限: 2011-1-1~2015-12-31
Research Theme:
Protein-Phosphoprotein Interaction in Cancer related Signaling
蛋白質與磷酸化蛋白質的相互作用在癌症信號傳導中的功能研究
Annual support: 1,600,000 NTD
2012 Academia Sinica Thematic Project
計畫編號: AS-101-TP-B02
補助期限: 2012-1-1~2014-12-31
Research Theme:
Roles of NIFK in Cancer (NIFK與癌症之關聯)
B02-2NIFK: Roles of NIFK phosphorylation and its binding with Ki67 in the cancer relevance
NIFK磷酸化及其與Ki67連結與癌症之關聯
Annual support: 8,000,000 NTD (for the program project)
MOST
計畫編號: 103-2113-M-001 -016 -MY3
補助期限: 2014-8-1~2017-7-31
Research Theme: How low-fidelity DNA polymerases catalyze non-Watson-Crick incorporation
低準確度DNA複製酶如何催化非Watson-Crick鹼對
每一年度金額: 2,500,000
Patents

      U.S. Provisional Application No. 61/515301
                Entitled: INFLUENZA VIRUS INHIBITORS THAT DISRUPT NUCLEOPROTEIN TRIMERIZATION
                Filing Date: August 4, 2011
General Publications

1. "Learning Is a Lifelong Process" in Reflections on Multiliterate Lives, Diane Dewhurst Belcher, Ulla Connor, Eds., Multilingual Matters, 2001.
Research Publications

The h-index is 46. The papers cited 46 times or more are bolded. Reviews are indicated.

1. "Terpenes and Sterols of Cunninghamia Konishii," Y. S. Cheng and M.-D. Tsai, Phytochemistry, 11, 2108-2109 (1972).
2. "Air Oxidation of α-Terpineol," M.-D. Tsai and Y. S. Cheng, J. Chinese Chem. Soc., 22, 149-155 (1974).
3. "Dye-sensitized Photooxidation of α-Terpineol," Y. S. Cheng, M.-D. Tsai, J. M. Fang and S. S. Hsu, Chemistry (The Chinese Chem. Soc., Taiwan, China), 8-11 (1975).
4. "Conformational Analysis of Pyridoxal Amino Acid Schiff's Bases," H. J. R. Weintraub, M.-D. Tsai, S. R. Byrn, C.-j. Chang and H. G. Floss, Int. J. Quantum Chem., QBS 10(S3), 99-105 (1976).
5. "Conformational analysis of pyridoxal Schiff's bases. Nuclear magnetic resonance studies of the conformations about the C4-C4', Cα-Cβ, and N-Cα bonds of the pyridoxal Schiff's bases of amino acids," M.-D. Tsai, S. R. Byrn, C.-j. Chang, H. G. Floss and H. J. R. Weintraub, Biochemistry 17, 3177-3182 (1978).
6. "Conformation-reactivity relationship for pyridoxal Schiff's bases. Rates of racemization and α-hydrogen exchange of the pyridoxal Schiff's bases of amino acids," M.-D. Tsai, H. J. R. Weintraub, S. R. Byrn, C.-j. Chang and H. G. Floss, Biochemistry, 17, 3183-3188 (1978).
7. "Stereochemistry and Mechanism of Reactions Catalyzed by Tryptophan Synthetase and its β2 Subunit," M.-D. Tsai, E. Schleicher, R. Potts, G. E. Skye and H. G. Floss, J. Biol. Chem., 253, 5344-5349 (1978).
8. "Stereochemistry and Mechanism of Reactions Catalyzed by Tryptophanase from Escherichia coli," J. C. Vederas, E. Schleicher, M.-D. Tsai and H. G. Floss, J. Biol. Chem., 253, 5350-5354 (1978).
9. "Stereochemistry of the β-Cyanoalanine Synthetase and S-Alkylcysteine Lyase Reactions," M.-D. Tsai, J. Weaver, H. G. Floss, E. E. Conn, R. K. Creveling and M. Mazelis, Arch. Biochem. Biophys., 190, 553-559 (1978).
10. "Stereochemistry of Enzymatic Transmethylation," H. G. Floss, L. Mascaro, M.-D. Tsai and R. W. Woodard, in Transmethylation (E. Usin, R. T. Borchardt and C. R. Creveling, Eds.), Elsevier North-Holland, New York (1979), pp. 135-141. (Review)
11. "Chiral Methyl Groups," H. G. Floss and M.-D. Tsai, Adv. Enzymol. 50, 243-302 (1979). (Review)
12. "Stereochemistry and Mechanism of Reactions Catalyzed by Indolyl-3-alkane α-Hydroxylase," M.-D. Tsai, H. G. Floss, H. J. Rosenfeld and J. Roberts, J. Biol. Chem., 254, 6437-6443 (1979).
13. "Stereochemical Course of the Transmethylation Catalyzed by Catechol O-Methyltransferase," R. W. Woodard, M.-D. Tsai, H. G. Floss, P. A. Cook and J. K. Coward, J. Biol. Chem., 255, 9124-9127 (1980).
14. "First Observation of Amino Acid Side Chain Dynamics in Membrane Proteins Using High Field Deuterium NMR Spectroscopy," R. A. Kinsey, A. Kintanar, M.-D. Tsai, R. L. Smith, N. Janes and E. Oldfield, J. Biol. Chem., 256, 4146-4149 (1981).
15. "Protein crystals, membrane proteins and membrane lipids. Recent advances in the study of their static and dynamic structures using nuclear magnetic resonance spectroscopic techniques." E. Oldfield, N. James, R. Kinsey, A. Kintanar, R. W. K. Lee, T. M. Rotngeb, S. Schramm, R. Skarjune, R. Smith and M.-D. Tsai, Biochem. Soc. Symp., 46, 155-181 (1981).
Papers from independent research:
16. "Use of 31P Nuclear Magnetic Resonance to Distinguish Bridge and Nonbridge Oxygens of 17O-enriched Nucleoside Triphosphates. Stereochemistry of Acetate Activation by Acetyl CoA Synthetase," M.-D. Tsai, Biochemistry 18, 1468-1472 (1979).
(This and a few subsequent papers were highlighted in Science 1984 in pdf.)
17. "Applicability of the 31P(17O) NMR Method in the Study of Enzyme Mechanism Involving Phosphorus," M.-D. Tsai, S. L. Huang, J. F. Kozlowski and C. C. Chang, Biochemistry 19, 3531-3536 (1980).
18. "Chirality at a Pro-pro-prochiral Phosphorus Center, Stereochemical Course of the 5'-Nucleotidase-Catalyzed Reaction," M.-D. Tsai and T. T. Chang, J. Am. Chem. Soc., 102, 5416-5418 (1980).
19. "Stereochemistry of the Hydrolysis of Adenosine 5'-Thiophosphate Catalyzed by Venom 5'-Nucleotidase," M.-D. Tsai, Biochemistry 19, 5310-5316 (1980).
20. "Does the Magnesium(II) Ion Interact with the α-Phosphate of ATP? An Investigation by 17O NMR," S.-L. Huang and M.-D. Tsai, Biochemistry 21, 951-959 (1982).
21. "Use of 31P(18O), 31P(17O), and 17O NMR Methods to Study Enzyme Mechanism Involving Phosphorus," M.-D. Tsai, Methods Enzymol. 87, 235-279 (1982).
22. "Phospholioids Chiral at Phosphorus. 1. Stereochemistry of Transphosphatidylation Catalyzed by Phospholipase D," K. Bruzik and M.-D. Tsai, J. Am. Chem. Soc. 104, 863-865 (1982).
23. "Phospholipids Chiral at Phosphorus. 2. Preparation, Property, and Application of Chiral Thiophospholipids," K. Bruzik, S. M. Gupte and M.-D. Tsai, J. Am. Chem. Soc. 104, 4682-4684 (1982).
24. "Phospholipids Chiral at Phosphorus. 3. Preparation and Spectral Properties of Chiral Thiophospholipids," K. Bruzik, R.-T. Jiang and M.-D. Tsai, Biochemistry 22, 2478-2486 (1983).
25. "Phospholipids Chiral at Phosphorus. 4. Could Membranes be Chiral at Phosphorus?" M.-D. Tsai, R.-T. Jiang and K. Bruzik, J. Am. Chem. Soc. 105, 2478-2480 (1983)
26. "Phospholipids Chiral at Phosphorus. Synthesis, Absolute Configurations and Applications." K. Bruzik, R.-T. Jiang and M.-D. Tsai, Phosphorus and Sulfur 18, 369-372 (1983).
27. "Effects of 17O and 18O on 31P NMR: Further Investigation and Applications," R. D. Sammons, P. A. Frey, K. Bruzik and M.-D. Tsai, J. Am. Chem. Soc. 105, 5455-5461 (1983). – This paper was featured in an one-page news article in Science 224, 377 (1984).
28. "NMR Methods Involving Oxygen Isotopes in Biophosphates," M.-D. Tsai and K. Bruzik, in Biological Magnetic Resonance, Vol. 5, L. J. Berliner and J. Reuben, Eds., Plenum Press, New York, pp. 129-181 (1983).
29. "Phospholipids Chiral at Phosphorus. 5. Synthesis and Configurational Analysis of Chiral [17O,18O]-Phosphatidylethanolamine." K. Bruzik and M.-D. Tsai, J. Am. Chem. Soc. 106, 747-754 (1984).
30. "Phospholipids Chiral at Phosphorus. 6. Synthesis of Chiral Phosphatidylcholine and Stereochemistry of Phospholipase D." K. Bruzik and M.-D. Tsai, Biochemistry 23, 1656-1661 (1984).
31. "Phospholipids Chiral at Phosphorus. 7. Absolute Configuration of Chiral Thiophospholipids and Stereochemistry of Phospholipase D." R.-T. Jiang, Y.-J. Shyy, and M.-D. Tsai, Biochemistry 23, 1661-1667 (1984).
32. "Phospholipids Chiral at Phosphorus. 8. Properties of Small Unilamellar Vesicles of Chiral Thiophosphatidylcholine." T.-C. Tsai, R.-T. Jiang and M.-D. Tsai, Biochemistry 23, 5564-5570 (1984).
33. "Stereochemistry of Biological Reactions at Proprochiral Centers." H. G. Floss, M.-D. Tsai, and R. W. Woodard, Topics in Stereochemistry, E. L. Eliel, N. L. Allinger and S. H. Wilen, Eds., John Wiley & Sons, pp. 253-321 (1984). (Review)
34. "Use of Chiral thiophosphates and the Stereochemistry of Enzymatic Phosphoryl Transfer." M.-D. Tsai, in 31P NMR: Principles and Applications, D. Gorenstein. Ed.. Academic Press, pp. 175-197 (1984). (Review)
35. "Phospholipids Chiral at Phosphorus. 9. Use of Chiral Thiophosphatidylcholine to Study the Metal-Binding Properties of Bee Venom Phospholipase A2," T.-C. Tsai, J. Hart, R.-T. Jiang, K. Bruzik and M.-D. Tsai, Biochemistry 24, 3180-3188 (1985).
36. "Metal-Nucleotide Interactions. 3. 17O, 31P and 1H NMR Studies on the Interactions of Sc(III), La(III) and Lu(III) with ATP," Y.-J. Shyy, T.-C. Tsai and M.-D. Tsai, J. Am. Chem. Soc. 107, 3478-3484 (1985).
37. "Mechanism of Adenylate Kinase. 1. Use of 17O NMR to Study the Binding Properties of Substrates." D. A. Wisner, C. Steginsky, Y.-J. Shyy and M.-D. Tsai, J. Am. Chem. Soc. 107, 2814-2815 (1985).
38. "Phospholipids Chiral at Phosphorus. 10. Use of Chiral Thiophospholipids to Study the Mechanism of Phospholipase A2," M.-D. Tsai, K. Bruzik, J. Hart, R.-T. Jiang, T. Rosario-Jansen, T.-C. Tsai and D. A. Wisner, in "Mechanisms of Enzymatic Reactions: Stereochemistry", P. A. Frey, ed., Elsevier, 115-126 (1986). (Review)
39. "Phospholipids Chiral at Phosphorus. 11. FT-IR Study on the Gel-Liquid Crystalline Transition of Chiral Thiophosphatidylcholine," S.-B. Chang, J. O. Alben, D. A. Wisner and M.-D. Tsai, Biochemistry 25, 3435-3440 (1986).
40. "Phospholipids Chiral at Phosphorus. 12. Configurational Effect on the Thermotropic Properties of Chiral Dipalmitoylthiophosphatidylcholine," D. A. Wisner, T. Rosario-Jansen and M.-D. Tsai, J. Am. Chem. Soc., 108, 8064-8068 (1986).
41. "Phospholipids Chiral at Phosphorus. 13. Stereochemical Comparison of Phospholipase A2, Lecithin-Cholesterol Acyl Transferase, and Platelet-Activating Factor," T. Rosario-Jansen, H. J. Pownall, J. P. Noel and M.-D. Tsai, Phosphorus and Sulfur 30, 601-604 (1987).
42. "Phospholipids Chiral at Phosphorus. 14. Stereochemical Effects on the Thermotropic Properties of Thiophosphatidylcholines and Thiosphingomyelins," M.-D. Tsai, K. S. Bruzik, D. Wisner and S.-H. Liu, in"Biophosphates and Their Analogues, Synthesis, Structure, Metabolism and Activity", K.S. Bruzik and W.J. Stec, Eds., Elsevier, pp. 561-570 (1987). (Review)
43. "Is the Binding of Mg2+ to Calmodulin Significant? An Investigation by 25Mg NMR," M.-D. Tsai, T. Drakenberg, E. Thulin and S. Forsen, Biochemistry, 26, 3635-3643 (1987).
44. "Magnesium Binding to Calcium-Binding Proteins: A Regulatory Function?" T. Drakenberg, S. Forsen, E. Thulin, and M.-D. Tsai, in Calcium-Binding Proteins in Health and Disease, Norman, A. W., Vanaman, T. C., and Means, A. R., Eds., Academic Press, pp. 430-432 (1987). (Review)
45. "Phospholipids Chiral at Phosphorus. 15. Steric Course of the Reactions Catalyzed by Phosphatidylserine Synthases from E. coli and Yeast," C. R. H. Raetz, G. M. Carman, W. Dowhan, R.-T. Jiang, W. Waszkuc, W. Loffredo and M.-D. Tsai, Biochemistry, 26 4022-4027 (1987).
46. "A Calorimetric Study of the Thermotropic Behavior of Pure Sphingomyelin Diastereomers," K. S. Bruzik and M.-D. Tsai, Biochemistry 26, 5364-5368 (1987).
47. "Mechanism of Adenylate Kinase. 2. Does ATP bind to the AMP Site?" Y. J. Shyy, G. Tian, and M.-D. Tsai, Biochemistry, 26, 6411-6415 (1987).
48. "Mechanism of Adenylate Kinase. 3. Use of Deuterium NMR to Show Lack of Correlation Between Local Substrate Dynamics and Local Binding Energy." C. R. Sanders II and M.-D. Tsai, J. Am. Chem. Soc. 110, 3323-3324 (1988).
49. "Steric Course of the Reaction Catalyzes by Phosphatidylserine Decarboxylase from E. coli". Z. No, C. R. Sanders II, W. Dowhan, and M.-D. Tsai, Bioorg. Chem., 16, 184-188 (1988).
50. "Phospholipids Chiral at Phosphorus. 16. Synthesis and Stereospecificity of Phosphorothioate Analogues of Platelet-Activating Factor." T. Rosario-Jansen, R.-T. Jiang, D. J. Hanahan, and M.-D. Tsai, Biochemistry, 27, 4619-4624 (1988).
51. "Phospholipids Chiral at Phosphorus. 17. Characterization of the Subgel Phase of Thiophosphatidylcholines by Use of X-Ray Diffraction, P-31 NMR, and FT-IR Spectroscopy". H. E. Sarvis, W. Loffredo,R. A. Dluhy, L. Hernqvist, D. A. Wisner, and M.-D. Tsai, Biochemistry, 27, 4625-4631 (1988).
52. "Mechanism of Adenylate Kinase. 4. Histidine-36 Is Not Directly Involved in Catalysis, But Protects Cysteine-25 and Stabilizes the Tertiary Structure." G. Tian, C. R. Sanders II, F. Kishi, A. Nakazawa, and M.-D. Tsai, Biochemistry, 27, 5544-5552 (1988).
53. "Use of Short-Chain Cyclopentano-phosphatidylcholines to Probe the Mode of Activation of Phospnolipase A2 from Bovine Pancreas and Bee Venom". G. L. Lin, J. Noel, W. Loffredo, H. Sable, and M.-D. Tsai, J. Biol. Chem., 263, 13208-13214 (1988).
54. "Phospholipase A2 Engineering: Design, Synthesis, and Expression of a Gene for Bovine (Pro)Phospholipase A2". J. P. Noel and M.-D. Tsai, J. Cellular Biochem. 40 (UCLA Symposium Series), 309-320 (1989).
55. "Phospholipids Chiral at Phosphorus. 18. Stereochemistry of Phosphatidylinositide-specific Phospholipase C". G. Lin and M.-D. Tsai, J. Am. Chem. Soc., 111, 3099-3101 (1989).
56. "Ligand-Protein Interactions Via NMR of Quadrupolar Nuclei". C. R. Sanders II and M.-D. Tsai, Methods. Enzymol. 177, 317-333 (1989). (Review)
57. "Mechanism of Adenylate Kinase. 5. Is There a Relationship Between Local Substrate Dynamics, Local Binding Energy, and the Catalytic Mechanism?" C. R. Sanders II, G. Tian and M.-D. Tsai, Biochemistry 28, 9028-9043 (1989).
58. "Phospholipids Chiral at Phosphorus. 19. Synthesis and Configurational Assignment of Phosphorothioate Analogues of Phosphatidylserine". W. M. Loffredo and M.-D. Tsai, Bioorg. Chem. 18, 78-84 (1990).
59. "Phospholipids Chiral at Phosphorus. 20. Stereochemical Mechanism of Reactions Catalyzed by Phosphatidylinositide-Specific Phospholipase C From Bacillus Cereus and Guinea Pig Uterus". G. H. Lin, C. F. Bennett, and M.-D. Tsai, Biochemistry 29, 2747-2757 (1990).
60. "Mechanism of Adenylate Kinase. 6. Are the Essential Lysines Essential?" G. Tian, H. Yan, R.-T. Jiang, F. Kishi, A. Nakazawa, and M.-D. Tsai, Biochemistry 29, 4296-4304 (1990).
61. "Phospholipids Chiral at Phosphorus. 22. Synthesis of Dioleoylthiophosphatidylcholine and Stereospecificity of Lecithin-Cholesterol Acyltransferase". T. Rosario-Jansen, H. Pownall, R.-T. Jiang, and M.-D. Tsai, Bioorg. Chem. 18, 179-184 (1990).
62. "A Novel Expression Vector for High-Level Synthesis and Secretion of Foreign Proteins in E. coli: Overproduction of Bovine Pancreatic Phospholipase A2." T. Deng, J. P. Noel, and M.-D. Tsai, Gene 93, 229-234 (1990).
63. "Phospholipase A2 Engineering. 3. Replacement of Lysine-56 by Neutral Residues Improves Catalytic Potency Significantly, Alters Substrate Specificity, and Clarifies the Mechanism of Interfacial Recognition". J. P. Noel, T. Deng, K. J. Kelly, and M.-D. Tsai, J. Am. Chem. Soc. 112, 3704-3706 (1990).
64. "Mechanism of Adenylate Kinase. 7. Structural and Functional Demonstration of Arginine-138 as a Key Catalytic Residue That Cannot be Replaced by Lysine". H. Yan, Z. Shi, and M.-D. Tsai, Biochemistry 29, 6385-6392 (1990).
65. "Phospholipase A2 Engineering. 4. Can the Active-Site Aspartate-99 Function Alone?" C. M. Dupureur, T. Deng, J.-G. Kwak, J. Noel, and M.-D. Tsai, J. Am. Chem. Soc. 112, 7074-7076 (1990).
66. "Phospholipids Chiral at Phosphorus. 23. Dramatic Effect of P-Chirality on the Deuterium NMR Properties of the Choline Head Group of Phospholipids in the Liquid Crystalline Phase". W. M. Loffredo, R.-T. Jiang, and M.-D. Tsai, Biochemistry 29, 10912-10918 (1990).
67. "Mechanism of Adenylate Kinase. 8. Critical Evaluation of the X-Ray Model and Assignment of the AMP Site." H. Yan, T. Dahnke, B. Zhou, A. Nakazawa, and M.-D. Tsai, Biochemistry 29, 10956-10964 (1990).
68. "Phospholipids Chiral at Phosphorus. 21. Phospholipase Stereospecificity at Phosphorus." K. Bruzik and M.-D. Tsai, Methods Enzymol. 197, 258-269 (1991). (Review)
69. "Phospholipids Chiral at Phosphorus. 24. Phosphorothioate Analogs of Phosphatidylinositol and Inositol 1,2-Cyclic Phosphate: Applications to the Mechanism of Phospholipase C." K. Bruzik, G. Lin, and M.-D. Tsai, ACS Symp. Series 463, 172-185 (1991). (Review)
70. "Mechanism of Adenylate Kinase. 9. Demonstration of Functional Relationship Between Aspartate 93 and Mg2+ by Site-Directed Mutagenesis and Proton, 31P, and 25Mg NMR." H. Yan and M.-D. Tsai, Biochemistry 30, 5539-5546 (1991).
71. "Mechanism of Adenylate Kinase. 10. Reversing Phosphorus Stereospecificity by Site-Directed Mutagenesis". R.-T. Jiang, T. Dahnke, and M.-D. Tsai, J. Am. Chem. Soc. 113, 5485-5486 (1991).
72. "Mechanism of Adenylate Kinase. 11. Site-Directed Mutagenesis Versus X-Ray and NMR." M.-D. Tsai and H. Yan, Biochemistry 30, 6806-6818 (1991). [This is a Perspectives in Biochemistry article.]
73. "Phospholipase A2 Engineering. 5. X-Ray Structural and Functional Evidence for the Interaction of Lysine-56 with Substrates." J. P. Noel, C. A. Bingman, T. Deng, C. M. Dupureur, K. J. Hamilton, R.-T. Jiang, J.-G. Kwak, C. Sekharudu, M. Sundaralingam, and M.-D. Tsai, Biochemistry 30, 11801-11811 (1991).
74. "Mechanism of Adenylate Kinase. 12. Prediction and Demonstration of Enhancement of Phosphorus Stereospecificity by Site-Directed Mutagenesis". T. Dahnke, R.-T. Jiang, and M.-D. Tsai, J. Am. Chem. Soc. 113, 9388-9389 (1991).
75. "Practical Synthesis of Enantiomerically Pure myo-Inositol Derivatives." K. S. Bruzik, J. Myers, and M.-D. Tsai, Tetrahedron Lett., 1009-1012 (1992).
76. "Phospholipase A2 Engineering. 6. Single Amino Acid Substitutions of Active Site Residues Convert the Rigid Enzyme to Highly Flexible Conformational States." C. M. Dupureur, Y. Li, and M.-D. Tsai, J. Am. Chem. Soc. 114, 2748-2749 (1992).
77. "Phospholipids Chiral at Phosphorus. 25. Stereochemical Mechanism for the Formation of Inositol 1-Phosphate Catalyzed by Phosphatidylinositide-Specific Phospholipase C." K. S. Bruzik, A. M. Morocho, D.-Y. Jhon, S. G. Rhee, and M.-D. Tsai, Biochemistry 31, 5183-5193 (1992).
78. "Mechanism of Adenylate Kinase. 13. Structural and Functional Roles of The Conserved Arginine-97 and Arginine-132." T. Dahnke, Z. Shi, H. Yan, R.-T. Jiang, and M.-D. Tsai, Biochemistry 31, 6318-6328 (1992).
79. "Phospholipase A2 Engineering. 7. Structural and Functional Roles of Highly Conserved Active Site Residues Tyrosine-52 and Tyrosine-73." C. M. Dupureur, B.-Z. Yu, M. Jain, J. P. Noel, T. Deng, Y. Li, I.-J. Byeon, and M.-D. Tsai, Biochemistry 31, 6402-6413 (1992).
80. "Efficient and Systematic Syntheses of Enantiomerically Pure and Regiospecifically Protected Myo-Inositols." K. S. Bruzik and M.-D. Tsai, J. Am. Chem. Soc. 114, 6361-6374 (1992).
81. "Interfacial Catalysis by Phospholipase A2: The Rate-Limiting Step for Enzymatic Turnover". M. K. Jain, B.-Z. Yu, J. Rogers, M. H. Gelb, M.-D. Tsai, E. K. Hendrickson, and H. S. Hendrickson, Biochemistry 31, 7841-7847 (1992).
82. "Phospholipase A2 Engineering. 9. The Structural and Functional Roles of Aromaticity and Hydrophobicity in the Conserved Phenylalanine-22 and Phenylalanine-106 Aromatic Sandwich." C. M. Dupureur, B.-Z. Yu, J. A. Mamone, M. K. Jain, and M.-D. Tsai, Biochemistry 31, 10576-10583 (1992).
83. "Crystal Structure of the Y52F,Y73F double mutant of phospholipase A2: increased hydrophobic interactions of the phenyl groups compensate for the disrupted hydrogen bonds of the tyrosines." C. Sekharudu, B. Ramakrishnan, B. Huang, R.-T. Jiang, C. M. Dupureur, M.-D. Tsai, and M. Sundaralingam, Protein Science 1, 1585-1594 (1992).
84. "Modification of a Bruker AM-600 Spectrometer for Double and Triple Resonance Three Dimensional and Four Dimensional Experiments Illustrated with Chicken Adenylate Kinase Resonance Assignments." E. S. Mooberry, A. S. Edison, F. Abildgaard, J. L. Markley, I.-J. L. Byeon, and M.-D. Tsai, Proceedings of the International Symposium on Spectroscopy and Structure of Molecules and Nuclei, N. R. Johnson, W. N. Shelton, and M. El-Sayed, Eds., World Scientific, pp. 375-380 (1992). (Review)
85. "Mechanism of Adenylate Kinase. 14. What Can be Learned from a Mutant Enzyme with Minor Perturbation in Kinetic Parameters?" Z.-T. Shi, I.-J. L. Byeon, R.-T. Jiang, ande M.-D. Tsai, Biochemistry 32, 6450-6458 (1993).
86. "Phospholipase A2 Engineering. 10. The Aspartate...Histidine Catalytic Diad Also Plays an Important Structural Role." Y. Li and M.-D. Tsai, J. Am. Chem. Soc. 115, 8523-8526 (1993).
87. "Mechanism of Adenylate Kinase. 15. 1H, 13C, and 15N NMR Assignments, Secondary Structures, and Substrate Binding Sites." I.-J. L. Byeon, H. Yan, A. S. Edison, E. S. Mooberry, F. Abildgaard, J. L. Markley, and M.-D. Tsai, Biochemistry 32, 12508-12521 (1993).
88. "Mechanism of Adenylate Kinase. 16. The Conserved Aspartates 140 and 141 Are Important for Transition State Stabilization Instead of Substrate-Induced Conformational Changes." T. Dahnke and M.-D. Tsai, J. Biol. Chem. 269, 8075-8081 (1994).
89. "Toward the Mechanism of Phospohatidylinositide-Specific Phospholipase C." K. S. Bruzik and M.-D. Tsai, Bioorg. & Med. Chem. 2, 49-72 (1994).
90. "Are D- and L-chiro-Phosphoinositides Substrates of Phosphatidylinositol-Specific Phospholipase C?" K. S. Bruzik, A. A. Hakeem, and M.-D. Tsai, Biochemistry 33, 8367-8374 (1994).
91. "A Small, High-Copy-Number Vector Suitable for Both in vitro and in vivo Gene Expressions." B. Huang, Z. Shi, and M.-D. Tsai, Gene 151, 143-145 (1994).
92. "Structure and Function of the Catalytic Site Mutant Asp 99 Asn of Phospholipase A2: Absence of the Conserved Structural Water." A. Kumar, C. Sekharudu, B. Ramakrishnan, C. M. Dupureur, H. Zhu, M.-D. Tsai, and M. Sundaralingam, Protein Science 3, 2082-2088 (1994).
93. "Phospholipase A2 Engineering. 12. Structural and Functional Roles of the Highly Conserved Active Site Residue Aspartate-49." Y. Li, B.-Z. Yu, H. Zhu, M. K. Jain, and M.-D. Tsai, Biochemistry 33, 14714-14722 (1994).
94. "Phospholipase A2 Engineering. 13. Conversion of Bovine Pancreatic Phospholipase A2 at a Single Site into a Competitor of Neurotoxic Phospholipase A2 by Site-Directed Mutagenesi. M.-C. Tzeng, C.-H. Yen, M.-J. Hseu, C. M. Dupureur, and M.-D. Tsai, J. Biol. Chem. 270, 2120-2123 (1995).
95. "Mechanism of Adenylate Kinase. 17. Manipulating Phosphorus Stereospecificity of Adenylate Kinase by Site-Directed Mutagenesis." M.-D. Tsai, R.-T. Jiang, T. Dahnke, and Z. Shi, Methods Enzymol. 249, 425-443 (1995). (Review)
96. "Mechanism of Adenylate Kinase. 18. The "Essential Lysine" Helps To Orient the Phosphates and the Active Site Residues to Proper Conformations." I.-J. L. Byeon, Z. Shi, and M.-D. Tsai, Biochemistry 34, 3172-3182 (1995).
97. "Phospholipase A2 Engineering. 14. Probing the Structural and Functional Roles of N-Terminal Residues with Site-Directed Mutagenesis, X-ray, and NMR." X. Liu, H. Zhu, B. Huang, J. Rogers, B.-Z. Yu, A. Kumar, M. K. Jain, M. Sundaralingam, and M.-D. Tsai, Biochemistry 34, 7322-7334 (1995).
98. "Immunogenicity and Conformational Properties of an N-linked Glycosylated Peptide Epitope of Human T-Lymphotropic Virus Type 1 (HTLV-1)." S. F. Conrad, I.-J. L Byeon, A. M. DiGeorge, M. D. Lairmore, M.-D. Tsai, and P. T. P. Kaumaya, Biomedical Peptides, Proteins, & Nucleic Acids 1, 83-92 (1995). (Review)
99. "Binding Proteins on Synaptic Membranes for Crotoxin and Taipoxin, 2 Phospholipases A2 with Neurotoxicity." M. C. Tzeng, C. H. Yen, M. J. Hseu, C. C. Tseng, M.-D. Tsai, and C. M. Dupureur, Toxicon 33, 451-457 (1995).
100. "Phospholipase A2 Engineering. 15. The Roles of Disulfide Bonds in Structure, Conformational Stability, and Catalytic Function.'' H. Zhu, C. M. Dupureur, X. Zhang, and M.-D. Tsai, Biochemistry 34, 15307-15314 (1995).
101. "Design, Synthesis and Biochemical Applications of Analogs of Phosphatidylinositol", N. K. Bhamare, Y. Wang, M.-D. Tsai, K. S. Bruzik, Phosphorus, Sulfur, and Silicon 109-110, 317-320 (1996).
102. "Structure-Function Relationship of Adenylate Kinase. 19. Glutamine-101 in AMP Specificity." S. Beichner, I.-J. L. Byeon, and M.-D. Tsai, PEPTIDES: Chemistry, Structure, and Biology, Kaumaya, P. T. P. and Hodges, R. S. (Eds.), Mayflower Scientific Ltd. (1996), Chapter 303, pp. 721-723. (Review)
103. "DNA Polymerase β: 1. Pre-Steady-State Kinetic Analysis and the Roles of Arginine-283 in Catalysis and Fidelity." B. G. Werneburg, J. Ahn, X. Zhong, R. J. Hondal, V. Kraynov, and M.-D. Tsai, Biochemistry 35, 7041-7050 (1996).
104. "Mechanism of Adenylate Kinase. 20. Probing the Importance of the Aromaticity in Tyrosine-95 and the Ring Size in Proline-17 with Unnatural Amino Acids." Z. Zhao, X. Liu, Z. Shi, L. Danley, B. Huang, R.-T. Jiang, and M.-D. Tsai, J. Am. Chem. Soc. 118, 3535-3536 (1996).
105. "Tumor Suppressor p16INK4A: 1. Structural Characterization of Wild-type and Mutant Proteins by NMR and Circular Dichroism." A. Tevelev, I.-J. L. Byeon, T. Selby, K. Ericson, H.-J. Kim, V. Kraynov, and M.-D. Tsai, Biochemistry 35, 9475-9487 (1996).
106. "Splase: A New Class llS Zinc-Finger Restriction Endonuclease with Specificity for Spl Binding Sites." B. Huang, C. J. Schaeffer, and M.-D. Tsai, J. Protein Chem. 15, 481-489 (1996).
107. "Synthesis of Inositol Phosphodiesters by Phospholipase C-Catalyzed Transesterification". K. S. Bruzik, Z. Guan, S. Riddle, and M.-D. Tsai, J. Am. Chem. Soc. 118, 7679-7688 (1996).
108. "Phospholipase A2 Engineering. 16. Deletion of the C-Terminus Segment Changes Substrate Specificity and Uncouples Calcium and Substrate Binding at the Zwitterionic Interface." B. Huang, B.-Z. Yu, J. Rogers, K. Sekar, M. Sundaralingam, M.-D. Tsai, and M. K. Jain, Biochemistry 35, 12164-12174 (1996).
109. "Binding Proteins on Synaptic Membranes for Certain Phospholipases A2 with Presynaptic Toxicity". M.-C. Tzeng, C.-H. Yen, and M.-D. Tsai, in "Natural Toxins II ", pp. 271-278, B. R. Singh and A. T. Tu, Eds., Plenum Press, New York (1996). [Also cite as Adv. Exp. Med. Biol. 391, 271-8 (1996).] (Review)
110. "Splase: The Design of a New Restriction Enzyme with Specificity for Sp1 Binding Sites." B. Huang, C. J. Schaeffer, and M.-D. Tsai, in Perspectives on Protein Engineering 3 (Geisow, M. J., ed), BIODIGM, Nottingham (1996). (Review)
111. "Effects of Glycosylation of a Peptide Epitope from HTLV-1 gp46 on Structure and Immunogenicity." S. F. Conrad, I.-J. L Byeon, A. M. DiGeorge, M. D. Lairmore, M.-D. Tsai, and P. T. P. Kaumaya, Pept.: Chem., Struct. Biol., Proc. Am. Pept. Symp., 14th, 448-9 (1996). (Review)
112. "DNA Polymerase β: 2. Structure-Fidelity Relationship from Pre-Steady-State Kinetic Analysis of All Possible Correct and Incorrect Base Pairs for Wild Type and R283A Mutant." J. Ahn, B. G. Werneburg, and M.-D. Tsai, Biochemistry 36, 1100-1107 (1997).
113. "DNA Polymerase β: 3. Analysis of the contribution of Tyrosine-271 and Asparagine-279 to Substrate Specificity and Fidelity of DNA Replication by Pre-steady-state Kinetics" V. S. Kraynov, B. G. Werneburg, X. Zhong, H. Lee, J. Ahn, and M.-D. Tsai, Biochem. J. 323, 103-111 (1997).
114. "Phospholipase A2 Engineering. 17. Structural and Functional Roles of the Highly Conserved Active Site Residue Aspartate-99." K. Sekar, B.-Z. Yu, J. Rogers, J. Lutton, X. Liu, X. Chen, M.-D. Tsai, M. Jain, & M. Sundaralingam, Biochemistry 36, 3104-3114 (1997).
115. "Synthesis of Enantiomerically Pure Phosphorothiolate Assay Substrate for Phosphatidylinositol-Specific Phospholipase C." Cornelia Mihai, Jan Mataka, Suzette Riddle, Ming-Daw Tsai, and Karol S. Bruzik, Bioorg. Med. Lett. 7, 1235-1238 (1997).
116. "PI-Specific Phospholipase C. 1. Kinetic and Stereochemical Evidence for an Interaction between Arginine-69 and the Phosphate Group of Phosphatidylinositol." Robert J. Hondal, Suzette R. Riddle, Alexander V. Kravchuk, Zhong Zhao, Karol S. Bruzik, and Ming-Daw Tsai, Biochemistry 36, 6633-6642 (1997).
117. "Natural Product Chemistry: From Plants to Human." Ming-Daw Tsai, J. Chin. Chem. Soc. 44, 183-186 (1997). (Review)
118. "Use of Unnatural Amino Acids to Probe the Importance of the Aromaticity in Tyrosine-95 and the Ring Size in Proline-17 in Adenylate Kinase." Z. Zhao, X. Liu, Z. Shi, B. Huang, R.-T. Jiang, and M.-D. Tsai, Youji Huaxue 17, 20-23 (1997).
119. "Mechanism of Phosphatidylinositol-Phospholipase C. 2. Reversal of a Thio Effect by Site-Directed Mutagenesis." Robert J. Hondal, Zhong Zhao, Karol S. Bruzik, and Ming-Daw Tsai, J. Am. Chem. Soc. 119, 5477-5478 (1997).
120. "DNA Polymerase β: 4. Multiple Conformational Changes in the Mechanism of Catalysis". Xuejun Zhong, Smita S. Patel, Brian G. Werneburg, and Ming-Daw Tsai, Biochemistry 36, 11891-11900 (1997).
121. "Phosphatidylinositol-Specific Phospholipase C. 3. Elucidation of the Catalytic Mechanism and Comparison with Ribonuclease A." Robert J. Hondal, Zhong Zhao, Suzette R. Riddle, Alexander V. Kravchuk, Hua Liao, Karol S. Bruzik, and Ming-Daw Tsai, J. Am. Chem. Soc. 119, 9933-9934 (1997).
122. "Structure of the Complex of Bovine Pancreatic Phospholipase A2 with a Transition-State Analogue." K. Sekar, A. Kumar, X. Liu, M.-D. Tsai, M. Gelb, and M. Sundaralingam, Acta Crystallogr. D. Biol. Crystallogr. 54, 334-341 (1998).
123. "1.72 Å Resolution Refinement of the Trigonal Form of Bovine Pancreatic Phospholipase A2." K. Sekar, A. Kumar, X. Liu, M.-D. Tsai, M. Gelb, and M. Sundaralingam, Acta Crystallogr. D. Biol. Crystallogr. 54, 342-346 (1998).
124. "DNA Polymerase β: 6. Effects of Gapped DNA Substrates on dNTP Specificity, Fidelity, Processivity, and Conformational Changes." Jinwoo Ahn, Vadim Kraynov, Xuejun Zhong, Brian G. Werneburg, and Ming-Daw Tsai, Biochem. J. 331, 79-87 (1998).
125. "DNA Polymerase β. 5. Dissecting the Functional Roles of the Two Metal Ions with Cr(III)dTTP" by Xuejun Zhong, Smita S. Patel, and Ming-Daw Tsai, J. Am. Chem. Soc. 120, 235-236 (1998).
126. "Tumor Suppressor p16INK4A: Determination of Solution Structure and Analyses of Its Interaction with Cyclin-Dependent Kinase 4". In-Ja L. Byeon, Junan Li, Karen Ericson, Thomas L. Selby, Anton Tevelev, Hee-Jung Kim, Paul O'Maille, and Ming-Daw Tsai, Molecular Cell 1, 421-431 (1998).
127. "Mechanism of PI-Specific Phospholipase C. 4. A Unified View of the Mechanism of Catalysis" Robert J. Hondal, Zhong Zhao, Alexander V. Kravchuk, Hua Liao, Suzette R. Riddle, Xiangjun Yue, Karol S. Bruzik, and Ming-Daw Tsai, Biochemistry 37, 4568-4580 (1998).
128. "Mechanism of PI-Specific Phospholipase C. 5. Revealed by Protein Engineering and Phosphorothioate Analogs of Phosphatidylinositol." Robert J. Hondal, Zhong Zhao, Alexander V. Kravchuk, Hua Liao, Suzette R. Riddle, Karol S. Bruzik, and Ming-Daw Tsai, in Phosphoinositides: Chemistry, Biochemistry and Biomedical Applications, K. S. Bruzik, Ed. ACS Symp. Ser. vol. 718, pp. 109-120 (1998). (Review)
129. "Cationic Residues 53 and 56 Control the Anion-Induced Interfacial k*cat-Activation of Pancreatic Phospholipase A2." Joseph Rogers, Bao-Zhu Yu, Ming-Daw Tsai, Otto Berg, and Mahendra K. Jain, Biochemistry 37, 9549-9556 (1998).
130. "Identification of a Novel Catalytic Triad with Dual Functions in Enzymatic Cleavage of the P-O Bond". Robert J. Kubiak, Robert J. Hondal, Xiangjun Yue, Ming-Daw Tsai, and Karol S. Bruzik, J. Am. Chem. Soc. 121, 488-489 (1999).
131. "Structures of the Catalytic Site Mutants D99A and H48Q and the Calcium Loop Mutant D49E of Phospholipase A2". K. Sekar, R. Biswas, Y. Li, M.-D. Tsai, and M. Sundaralingam, Acta Crystallogr. D55, 443-447 (1999).
132. "Structural Analysis of Phospholipase A2 from Functional Perspective. 1. Functionally Relevant Solution Structure and Roles of the Hydrogen-Bonding Network". Chunhua Yuan, In-Ja L. Byeon, Yishan Li, and Ming-Daw Tsai, Biochemistry 38, 2909-2918 (1999).
133. "Structural Analysis of Phospholipase A2 from Functional Perspective. 2. Characterization of a Molten Globule-like State Induced by Site-Specific Mutagenesis". Chunhua Yuan, In-Ja L. Byeon, Ming Jye Poi, and Ming-Daw Tsai, Biochemistry 38, 2919-2929 (1999).
134. "Tumor Suppressor INK4: 3. Determination of the Solution Structure of p18INK4C and Demonstration of the Functional Significance of Loops in p18INK4C and p16INK4A." Junan Li, In-Ja L. Byeon, Karen Ericson, Ming Jye Poi, Paul O’Maille, Thomas Selby, and Ming-Daw Tsai, Biochemistry 38, 2930-2940 (1999).
135. "Contributions of Residues of Pancreatic Phospholipase A2 to Interfacial Binding, Catalysis and Activation." Bao-Zhu Yu, Joseph Rogers, Ming-Daw Tsai, Charles Pidgeon, and Mahendra K. Jain, Biochemistry 38, 4875-4884 (1999).
136. "Nucleoside Monophosphate Kinases: Structure, Mechanism, and Substrate Specificity." Honggao Yan and Ming-Daw Tsai, Adv. Enzymol. And Related Area of Mol. Biol. 73: Mechanism of Enzyme Action, D. L. Purich, Ed., pp. 103-134 (1999). (Review)
137. "Pancreatic Phospholipase A2: New Views on Old Issues". Chunhua Yuan and Ming-Daw Tsai, BBALIP – Special Thematic Issue on “Lipids in the Center”, BBA-Mol. Cell. Biol. L 1441, 215-222 (1999).
138. "Tumor Suppressor INK4: Comparisons of Conformational Properties between p16INK4A and p18INK4C." Chunhua Yuan, Junan Li, Thomas L. Selby, In-Ja L. Byeon, and Ming-Daw Tsai, J. Mol. Biol. 294, 201-211 (1999).
139. "Structure and Function of A New Phosphopeptide-binding Domain Containing the FHA2 of Rad53" by Hua Liao, In-Ja L. Byeon, and Ming-Daw Tsai, J. Mol. Biol. 294, 1041-1049 (1999).
140. "Tumor Suppressor INK4: Quantitative Structure-Function Analyses of p18INK4C as an Inhibitor of Cyclin-Dependent Kinase 4." Junan Li, Ming Jye Poi, Dongyan Qin, Thomas L. Selby, In-Ja Byeon, and Ming-Daw Tsai, Biochemistry 39, 649-657 (2000).
141. "Structure-Function Relationship of the INK4 Family of Tumor Suppressors". Junan Li, In-Jia L. Byeon, Ming Jye Poi, Karen Ericson, Thomas Selby, Paul O’Maille, Dongyan Qin, and Ming-Daw Tsai, in DNA Alterations in Cancer: Genetic and Epigenetic Changes, Melanie Ehrlich, Ed., BioTechniques Books, Eaton Publishing, pp. 71-84 (2000). (Review)
142. "Tumor Suppressor INK4: Refinement of p16INK4A Structure and Determination of p15INK4B Structure by Comparative Modeling and NMR Data". Chunhua Yuan, Thomas L. Selby, Junan Li, In-Ja Byeon, and Ming-Daw Tsai, Protein Science 9, 1120-1128 (2000).
143. "Kinetic Isotope Effects and Stereochemical Studies on a Ribonuclease Model: Hydrolysis Reactions of Uridine-3’-Nitrophenyl Phosphate". Alvan C. Hengge, Karol S. Bruzik, Aleksandra E. Tobin, W. W. Cleland, and Ming-Daw Tsai, Bioorg. Chem. 28, 119-133 (2000).
144. "Structural Basis of the Anionic Interface Preference and kcat Activation of Pancreatic Phospholipase A2". Bao-Zhu Yu, Ming Jye Poi, U.A. Ramagopal, Rinku Jain, S. Ramakumar, Otto Berg, Ming-Daw Tsai, K. Sekar, and Mahendra Kumar Jain, Biochemistry 39, 12312-12323 (2000).
145. "Structure and Specificity of the Interaction between the FHA2 Domain of Rad53 and Phosphotyrosyl Peptides". Peng Wang, In-Ja L. Byeon, Hua Liao, Kirk Beebe, Suganya Yongkiettrakul, Dehua Pei, and Ming-Daw Tsai, J. Mol. Biol. 302, 927-940 (2000).
146. "Structure of the FHA1 Domain of Yeast Rad53 and Identification of Binding Sites for both FHA1 and Its Target Protein Rad9". Hua Liao, Chunhua Yuan, Mei-I Su, Suganya Yongkiettrakul, Dongyan Qin, Hongyuan Li, In-Ja L. Byeon, Dehua Pei, and Ming-Daw Tsai, J. Mol. Biol. 304, 941-951 (2000).
147. "DNA Polymerase β: Contributions of Template-Positioning and dNTP Triphosphate-Binding Residues to Catalysis and Fidelity." Vadim S. Kraynov, Alexander K. Showalter, Jia Liu, Xuejun Zhong and Ming-Daw Tsai, Biochemistry 39, 16008-16015 (2000).
148. "Somatic INK4a-ARF Locus Mutations: A Significant Mechanism of Gene Inactivation in Squamous Cell Carcinomas of the Head and Neck." Ming J. Poi, Thomas Yen, Junan Li, Huijuan Song, Jas C. Lang, Dave E. Schuller, Dennis K. Pearl, Bruce C. Casto, Ming-Daw Tsai, and Christopher M. Weghorst, Molecular Carcinogenesis 30, 26-36 (2001).
149. "A DNA Polymerase with Specificity for Five Base Pairs". Alexander K. Showalter and Ming-Daw Tsai, J. Am. Chem. Soc. 123, 1776-1777 (2001).
(This paper has been featured in Chemical & Engineering News, March 12, 2001, p.60-61.)
150. "Insight into the Catalytic Mechanism of DNA Polymerase β: Structures of Intermediate Complexes". Joseph W. Arndt, Weimin Gong, Xuejun Zhong, Alexander K. Showalter, Jia Liu, Christopher A. Dunlap, Zheng Lin, Chad Paxson, Ming-Daw Tsai, and Michael K. Chan, Biochemistry 40, 5368 -5375 (2001).
151. "Involvement of Arg…Asp…His Catalytic Triad in Enzymatic Cleavage of the Phosphodiester Bond". Robert J. Kubiak, Xiangjun Yue, Robert J. Hondal, Cornelia Mihai, Ming-Daw Tsai, and Karol S. Bruzik, Biochemistry 40, 5422-5432 (2001).
152. "Mechanism of PI-PLC: Origin of Unusually High Nonbridging Thio Effects". Alexander V. Kravchuk, Li Zhao, Robert J. Kubiak, Karol S. Bruzik, and Ming-Daw Tsai, Biochemistry 40, 5433-5439 (2001).
153. "DNA Polymerase β: Pre-steady-State Kinetic Analyses of dATPαS Stereoselectivity and Alteration of the Stereoselectivity by Varying Metal Ions and by Site-Directed Mutagenesis." Jia Liu and Ming-Daw Tsai, Biochemistry 40, 9014-9022 (2001).
154. "Interfacial Enzymology: The Secreted Phospholipase A2-Paradigm". Otto G. Berg, Michael Gelb, Ming-Daw Tsai, and Mahendra K. Jain, Chemical Reviews 101, 2613-2653 (2001). (Review)
155. "Solution Structure of a Viral DNA Polymerase X and Evidence for a Mutagenic Function" by Alexander K. Showalter, In-Ja L. Byeon, Mei-I Su, and Ming-Daw Tsai, Nature Structural Biology 8, 942-946 (2001).
(This paper has been featured in News & Views of NSB.)
156. "Solution Structures of Two FHA1-Phosphothreonine Peptide Complexes Provide Insight into the Structural Basis of the Ligand Specificity of FHA1 from Yeast Rad53." Yuan, C., Yongkiettrakul, S., Byeon, I.-J. L., Zhou, S., & Tsai, M.-D., J. Mol. Biol. 314, 563-575 (2001).
157. "Solution Structure of the Yeast Rad53 FHA2 Complexed with a Phosphothreonine Peptide pTXXL: Comparison with the Structures of FHA2-pYXL and FHA1-pTXXD Complexes." Byeon, I. -J. L., Yongkiettrakul, S., & Tsai, M.-D. J. Mol. Biol. 314, 577-588 (2001).
158. "Novel Insights into the INK4-CDK4/6-Rb Pathway: Counter Action of Gankyrin Against INK4 Proteins Regulates the CDK4-Mediated Phosphorylation of Rb". Junan Li and Ming-Daw Tsai, Biochemistry 41, 3977-3983 (2002).
159. "FHA: A Signal Transduction Domain with Diverse Specificity and Function". Ming-Daw Tsai, Structure 10, 887-888 (2002). (Review)
160. "Structure-based Combinatorial Protein Engineering (Scope)" by Paul E. O’Maille, Marina Bakhtina, and Ming-Daw Tsai, J. Mol. Biol. 321, 677-691 (2002).
161. "A Reexamination of the Nucleotide Incorporation Fidelity of DNA Polymerases". Alexander K. Showalter and Ming-Daw Tsai, Biochemistry 41, 10571-10576 (2002). [New Concepts in Biochemistry]
(This work has been featured in Chemical & Engineering News, May 13, 2002, p.44.)
162. "Use of 2-Aminopurine and Tryptophan Fluorescence as Probe in Kinetic Analyses of DNA Polymerase β". Christopher A. Dunlap and Ming-Daw Tsai, Biochemistry 41, 11226-11235 (2002).
163. "Observation of Additional Calcium Ion in the Crystal Structure of the Triple Mutant K56,120,121M of Bovine Pancreatic Phospholipase A2". V. Rajakannan, M. Yogavel, Ming-Jye Poi, A. Jeya Prakash, J. Jeyakanthan, D. Velmurugan, Ming-Daw Tsai and K. Sekar, J. Mol. Biol. 324, 755-762 (2002).
164. "A Novel Calcium-Dependent Bacterial Phosphatidylinositol-Specific Phospholipase C Displaying Unprecedented Magnitudes of Thio Effect, Inverse Thio Effect, and Stereoselectivity". Li Zhao, Yinghui Liu, Karol S. Bruzik, and Ming-Daw Tsai, J. Am. Chem. Soc. 125, 22-23 (2003).
165. "Engineering a Catalytic Metal Binding Site into a Calcium-independent Phosphatidylinositol-Specific Phospholipase C Leads to Enhanced Stereoselectivity". Alexander V. Kravchuk, Li Zhao, Karol S. Bruzik, and Ming-Daw Tsai, Biochemistry 42, 2422-2430 (2003).
166. "Application of BrØnsted-type LFER in the Study of the Phospholipase C Mechanism". Cornelia Mihai, Alexander V. Kravchuk, Ming-Daw Tsai and Karol S. Bruzik, J. Am. Chem. Soc. 115, 3236-3243 (2003).
167. "Expression and Characterization of Syrian Golden Hamster p16, a Homologue of Human Tumor Suppressor p16INK4A." Junan Li, Dongyan Qin, Thomas J. Knobloch, Ming-Daw Tsai, Christopher M. Weghorst, W. Scott Melvin, and Peter Muscarella, Biochem. Biophys. Res. Commun. 304, 241-247 (2003).
168. "Interaction of Monodisperse Anionic Amphiphiles with the i-Face of Secreted Phospholipase A2". Bao-Zhu Yu, Rafael Apitz-Castro, Ming-Daw Tsai, and Mahendra K. Jain, Biochemistry 42, 6293-6301 (2003).
169. "A Low-Barrier Hydrogen Bond Between Histidine of Secreted Phospholipase A2 and a Transition State Analog Inhibitor". Ming Jye Poi, John W. Tomaszewski, Chunhua Yuan, Christopher A. Dunlap, Niels H. Andersen, Michael H. Gelb, and Ming-Daw Tsai, J. Mol. Biol. 329, 997-1009 (2003).
170. "Direct Binding of the N-terminus of HTLV-1 Tax Oncoprotein to Cyclin-dependent Kinase 4 Is a Dominant Path to Stimulate the Kinase Activity". Junan Li, Hongyuan Li, and Ming-Daw Tsai, Biochemistry 42, 6921-6928 (2003).
171. "Biocatalysis and Biotransformation Enzymology in the Genomic Era – Editorial Overview." Tadhg P. Begley and Ming-Daw Tsai, Curr. Opin. Chem. Biol. 7, 228-229 (2003). (Review)
172. "Diverse but Overlapping Functions of the Two Forkhead-associated (FHA) Domains in Rad53 Checkpoint Kinase Activation." Brietta L. Pike, Suganya Yongkiettrakul, Ming-Daw Tsai, and Jorg Heierhorst, J. Biol. Chem. 278, 30421-30424 (2003).
173. "Crystal Structures of the Free and Anisic Acid Bound Triple Mutant of Phospholipase A2". K. Sekar, S. Vaijayanthi Mala, M. Yogavel, D. Velmurugan, Ming-Jye Poi, B. S. Vishwanath, T. V. Gowda, A. Arokia Jeyaprakash and M.-D. Tsai, J. Mol. Biol. 333, 367-376 (2003).
174. "Identification of Potential Binding Sites for the FHA Domain of Human Chk2 by in vitro Binding Studies." Dongyan Qin, Hyun Lee, Chunhua Yuan, Yong Ju, and Ming-Daw Tsai, Biochem. Biophys. Res. Commun. 311, 803-808 (2003).
175. "An NF-κB-Specific Inhibitor, IκBα, Binds to and Inhibits Cyclin-Dependent Kinase 4". Junan Li, Sang Hoon Joo, and Ming-Daw Tsai, Biochemistry 42, 13476-13483 (2003).
176. "Structure of human Ki67 FHA domain and its binding to a phosphoprotein fragment from hNIFK reveal unique recognition sites and new views to the structural basis of FHA domain functions." Hongyuan Li, In-Ja L. Byeon, Yong Ju, and Ming-Daw Tsai, J. Mol. Biol. 335, 371-381 (2004).
177. "Frequent p16INK4A/CDKN2A Alterations in Chemically Induced Syrian Golden Hamster Pancreatic Tumors". Junan Li, Christopher M. Weghorst, Masahiro Tsutsumi, Ming J. Poi, Thomas J. Knobloch, Bruce C. Casto, W. Scott Melvin, Ming-Daw Tsai, and Peter Muscarella, Carcinogenesis 25, 263-268 (2004).
178. "The Ligand Specificity of Yeast Rad53 FHA Domains at the +3 Position Is Determined by Nonconserved Residues" by Suganya Yongkiettrakul, In-Ja L. Byeon, and Ming-Daw Tsai, Biochemistry 43, 3862-3869 (2004).
179. "Mdt1, a Novel Rad53 FHA1 Domain-Interacting Protein, Modulates DNA Damage Tolerance and G2/M Cell Cycle Progression in Saccharomyces cerevisiae". Brietta L. Pike, Suganya Yongkiettrakul, Ming-Daw Tsai, and Jorg Heierhorst. Mol. Cell. Biol. 24, 2779-2788 (2004).
180. "The Nuclear Protein p34SEI-1 Regulates the Kinase Activity of Cyclin-dependent Kinase 4 in a Concentration-dependent Manner." Junan Li, W. Scott Melvin, Ming-Daw Tsai, and Peter Muscarella, Biochemistry 43, 4394-4399 (2004).
181. "Gene Library Synthesis by Structure-based Combinatorial Protein Engineering (SCOPE)." Paul E. O’Maille, Ming-Daw Tsai, and Joseph P. Noel, Methods in Enzymology 388, 75-91 (2004).
182. "The Catalytic Role of Aspartate in a Short Strong Hydrogen Bond of the Asp274...His32 Catalytic Dyad in Phosphatidylinositol-specific Phospholipase C Can Be Substituted by a Chloride Ion." Li Zhao, Hua Liao, and Ming-Daw Tsai, J. Biol. Chem. 279, 31995-32000 (2004).
183. "Solution Structure of Human Oncogenic Protein Gankyrin Containing Seven Ankyrin Repeats and Analysis of Its Structure-function Relationship". Chunhua Yuan, Junan Li, Anjali Mahajan, Ming Jye Poi, In-Ja L. Byeon, and Ming-Daw Tsai, Biochemistry 43, 12152-12161 (2004).
184. "Stereochemistry in Understanding Enzyme Kinetics and Mechanism." Ming-Daw Tsai, Li Zhao, and Brandon Lamarche, Encyclopedia in Biological Chemistry 2, 45-50 (2004). (Review)
185. "Atomic resolution (0.97Å) structure of the triple mutant (K53,56,121M) of bovine pancreatic phospholipase A2". K. Sekar, V. Rajakannan, D. Gayathri, D. Velmurugan, M.-J. Poi, M. Dauter, Z. Dauter and M.-D. Tsai, Acta Cryst. F61, 3-7 (2005).
186. "Unusual Four-bond Secondary H/D Isotope Effect Supports a Short-Strong Hydrogen Bond between Phospholipase A2 and a Transition State Analogue Inhibitor." Chunhua Yuan, Shengjiang Tu, Michael H. Gelb, and Ming-Daw Tsai, Biochemistry 44, 4748-4754 (2005).
187. "Use of Viscogens, dNTPαS and Rhodium(III) as Probes in Stopped-Flow Experiments to Obtain New Evidence for the Mechanism of Catalysis by DNA Polymerase β." Marina Bakhtina, Soojin Lee, Yu Wang, Chris Dunlap, Brandon Lamarche, and Ming-Daw Tsai, Biochemistry 44, 5177-5187 (2005).
188. "An Error-Prone Viral DNA Ligase." Brandon Lamarche, Alex Showalter, and Ming-Daw Tsai, Biochemistry 44, 8408-17 (2005).
189. "X-ray structure of the R69D phosphatidylinositol-specific phospholipase C enzyme: insight into the role of calcium and surrounding amino acids in active site geometry and catalysis." D. Apiyo, Li Zhao, Ming-Daw Tsai, and Thomas L. Selby, Biochemistry 44, 9980-9989 (2005).
190. "Dissection of CDK4-binding and Transactivation Activities of p34SEI-1 and Comparison between Functions of p34SEI-1 and p16INK4. Junan Li, Peter Muscarella, Sang Hoon Joo, Thomas, J. Knobloch, W. Scott Melvin, Christopher, M. Weghorst, and Ming-Daw Tsai, Biochemistry 44, 13246-13256 (2005).
191. "FHA Domain-Ligand Interactions: Importance of Integrating Chemical and Biological Approaches". Anjali Mahajan, Chunhua Yuan, Brietta L. Pike, Jorg Heierhorst, Chi-Fon Chang, and Ming-Daw Tsai, J. Am. Chem. Soc. 127, 14572-14573 (2005).
192. "Sequential Phosphorylation and Multisite Interactions Characterize Specific Target Recognition by the FHA Domain of Ki-67", by In-Ja L. Byeon, Hongyuan Li, Haiyan Song, Angela M. Gronenborn, and Ming-Daw Tsai, Nature Structural and Molecular Biology 12, 987-993 (2005).
193. "Atomic Resolution Structure of the Double Mutant (K53,56M) of Bovine Pancreatic Phospholipase A2". K. Sekar, M. Yogavel, D. Velmurugan, R.Krishna, M.-J. Poi, Z. Dauter, M. Dauter and M.-D. Tsai, Acta Cryst. F62, 1-5 (2006).
194. "Contributions of an Endonuclease IV Homologue to DNA Repair in the African Swine Fever Virus". B. Lamarche and M.-D. Tsai, Biochemistry 45, 2790-2803 (2006).
195. "Mechanistic Comparison of High-fidelity and Error-prone DNA Polymerases and Ligases Involved to DNA Repair". Brandon Lamarche, Alexander K. Showalter, Marina Bakhtina, Mei-I Su, Kuo-Hsiang Tang, and Ming-Daw Tsai, Chemical Reviews 106, 340-360 (2006).
196. "Third Calcium Ion Found in an Inhibitor Bound Phospholipase A2". K. Sekar, D. Gayathri, D. Velmurugan, J. Jeyakanthan, T. Yamane, M.-J. Poi, and M.-D. Tsai, Acta Cryst. D62, 392-397 (2006).
197. "Suggestive Evidence for the Involvement of Second Calcium and Surface Loop in Interfacial Binding: Monoclinic and Trigonal Crystal Structures of A Quadruple Mutant of Phospholipase A2." K. Sekar, M. Yogavel, D. Velmurugan, M.-J. Poi, Z. Dauter, and M.-D. Tsai, Acta Cryst. D62, 717-724 (2006).
198. Glycopeptide Biosynthesis: Dbv21/Orf2* from dbv/tcp Gene Clusters Are NAc-Glm Teicoplanin Pseudoaglycone Deacetylases and Orf15 from cep Gene Cluster Is a Glc-1-P Thymidyltransferase". Jin-Yuan Ho, Yu-Ting Huang, Chang-Jer Wu, Yi-Shan Li, Ming-Daw Tsai, and Tsung-Lin Li, J. Am. Chem. Soc. 128, 13694-13695 (2006).
199. "ASFV DNA Polymerase X Is Extremely Error-Prone under Diverse Assay Conditions and within Multiple DNA Sequence Contexts." Brandon J. Lamarche, Sandeep Kumar, and Ming-Daw Tsai, Biochemistry 45, 14826-14833 (2006).
200. "Ankyrin repeat: a unique motif mediating protein-protein interactions". Junan Li, Anjali Mahajan, and Ming-Daw Tsai, Biochemistry 45, 15168-15178 (2006).
201. "Use of Damaged DNA and dNTP Substrates by the Error-Prone DNA Polymerase X from African Swine Fever Virus". Sandeep Kumar, Brandon J. Lamarche, and Ming-Daw Tsai, Biochemistry 46, 3814-3825 (2007).
202. "Human DNA Ligase IV and the Ligase IV/XRCC4 Complex: Analysis of Nick Ligation Fidelity." Yu Wang, Brandon J. Lamarche, and Ming-Daw Tsai, Biochemistry 46, 4962-4976 (2007).
203. "A Unified Kinetic Mechanism Applicable to Multiple DNA Polymerases". Marina Bakhtina, Michelle P. Roettger, Sandeep Kumar, and Ming-Daw Tsai, Biochemistry 46, 5463-5472 (2007).
204. "Identification of Histone Demethylases in Sacchromyces cerevisiae". Shengjiang Tu, Esther M. M. Bulloch, Lanhao Yang, Chen Ren, Pang-Hung Hsu, Wei-Chieh Huang, Chein-Hung Chen, Chung-Lin Liao, Hui-Ming Yu, Wan-Sheng Lo, Michael A. Freitas, Ming-Daw Tsai, J. Biol. Chem. 282, 14262-14271 (2007).
205. "Human p16γ, a novel transcriptional variant of p16INK4A, coexpresses with p16INK4A in cancer cells and inhibits cell-cycle progression". You-Chin Lin, Mitchell B. Diccianni, Youngjin Kim, Hsin-Hung Lin, Chien-Hsin Lee, Ruey-Jen Lin, Sang Hoon Joo, Junan Li, An-Suei Yang, Huan-Hsien Kuo, Ming-Daw Tsai, Alice L. Yu, Oncogene 26, 7017-7027 (2007).
206. "Investigation of the Conformational States of Wzz and the Wzz-O-antigen Complex under Near-Physiological Conditions". Kuo-Hsiang Tang, Hongjie Guo, Wen Yi, Ming-Daw Tsai, and Peng George Wang, Biochemistry 46, 11744-11752 (2007).
207. "Dissection of Protein-Protein Interaction and CDK4 Inhibition in the Oncogenic versus Tumor Suppressing Functions of Gankyrin and P16." Anjali Mahajan, Yi Guo, Christopher M. Weghorst, Chunhua Yuan, Ming-Daw Tsai, Junan Li, J. Mol. Biol. 373, 990-1005 (2007).
208. "A Unique FMN-linked Primary Alcohol Oxidase for Glycopeptide A40926 Maturation". Yi-Shan Li, Jin-Yuan Ho, Chia-Chi Huang, Syue-Yi Lyu, Chun-Yen Lee, Yu-Ting Huang, Chang-Jer Wu, Hsiu-Chien Chan, Chuan-Jiuan Huang, Ning-Shian Hsu, Ming-Daw Tsai, and Tsung-Lin Li, J. Am. Chem. Soc. 129, 13384-13385 (2007).
209. "Identification of in Vivo Phosphorylation Sites and Their Functional Significance in the Sodium Iodide Symporter". Douangsone D. Vadysirisack, Eric S.-W. Chen, Zhaoxia Zhang, Ming-Daw Tsai, Geen-Dong Chang, and Sissy M. Jhiang, J. Biol. Chem. 282, 36820-8 (2007).
210. "Solution Structures of 2:1 and 1:1 DNA Polymerase-DNA Complexes Probed by Ultracentrifugation and Small-Angle X-ray Scattering". Kuo-Hsiang Tang, Marc Niebuhr, Ann Aulabaugh, and Ming-Daw Tsai, Nucleic Acids Res. 36, 849-860 (2008).
211. "The ARID domain of the H3K4 demethylase RBP2 binds to a DNA CCGCCC motif". Shengjiang Tu, Yu-Ching Teng, Chunhua Yuan, Ying-Ta Wu, Meng-Yu Chan, An-Ning Cheng, Po-Hsun Lin, Li-Jung Juan, and Ming-Daw Tsai, Nature Struc. Mol. Biol. 15, 419-421 (2008).
212. "Structure and Function of 2:1 DNA Polymerase•DNA Complexes". Kuo-Hsiang Tang and Ming-Daw Tsai, J. Cellular Physiology 216, 315-320 (2008). (Review).
213. "Mismatched dNTP Incorporation by DNA Polymerase β Does Not Proceed via Globally Different Conformational Pathways". Kuo-Hsiang Tang, Marc Niebuhr, Chang-Shung Tung, Hsiu-chien Chan, Chia-Cheng Chou, and Ming-Daw Tsai, Nucleic Acids Res. 36, 2948-2957 (2008). [Faculty of 1000]
214. "Diphosphothreonine-specific interaction between an SQ/TQ cluster and an FHA domain in the Rad53-Dun1 kinase cascade". Hyun Lee, Chunhua Yuan, Andrew Hammet, Anjali Mahajan, Eric S.-W. Chen, Ming-Ru Wu, Mei-I Su, Jörg Heierhorst, Ming-Daw Tsai, Mol. Cell 30, 767-778 (2008). [Faculty of 1000]
(This paper has been featured in Science, Science Signaling, and Mol Cell.)
215. "Altered Order of Substrate Binding by DNA Polymerase X from African Swine Fever Virus". Sandeep Kumar, Marina Bakhtina, and Ming-Daw Tsai, Biochemistry 47, 7875-7887 (2008).
216. "Mismatched and Matched dNTP Incorporation by DNA Polymerase β Proceed via Analogous Kinetic Pathways". Michelle P. Roettger, Marina Bakhtina, and Ming-Daw Tsai, Biochemistry 47, 9718-9727 (2008).
217. "Structure and Function of the Phosphothreonine-Specific FHA Domain". Anjali Mahajan, Chunhua Yuan, Hyun Lee, Eric S.-W. Chen, Pei-Yu Wu, and Ming-Daw Tsai, Science Signaling 1, re12 (2008).(Review)
218. "Humoral immunity against capsule polysaccharide protects the host from magA+ Klebsiella pneumoniae-induced lethal disease by evading Toll-Like Receptor 4 signaling". Ming-Fang Wu, Chih-Ya Yang, Tzu-Lung Lin, Jin-Town Wang, Feng-Ling Yang, Shih-HsiungWu, Bor-Shen Hu, Teh-Ying Chou, Ming-Daw Tsai, Chi-Hung Lin, Shie-Liang Hsieh, Infect. Immun. 77, 615-621 (2009).
219. "AMP-Activated Protein Kinase Functionally Phosphorylates Endothelial Nitric Oxide Synthase Ser633". Zhen Chen, I-Chen Peng, Wei Sun, Mei-I Su, Pang-Hung Hsu, Yi Fu, Yi Zhu, Kathryn DeFea, Songqin Pan, Ming-Daw Tsai, and John Y-J. Shyy, Circulation Res. 104, 496-505 (2009). (IF: 9.989)
220. "Contribution of the Reverse Rate of the Conformational Step to Polymerase β Fidelity". Marina Bakhtina, Michelle P. Roettger, and Ming-Daw Tsai, Biochemistry 48(14), 3197-3208 (2009)
221. "Comparisons of the Conformational Stability of Cyclin-dependent Kinase (CDK) 4-interacting Ankyrin Repeat (AR) Proteins". Yi Guo, Anjali Mahajan, Chunhua Yuan, Sang Hoon Joo, Christopher M. Weghorst, Ming-Daw Tsai, and Junan Li, Biochemistry 48(19), 4050-4062 (2009).
222. "Trans-Cyclization of Phosphatidylinositol Catalyzed by Phospholipase C from Streptomyces antibioticus". Chuan Bai, Li Zhao, Mario Rebecchi, Ming-Daw Tsai, and Karol S. Bruzik, J. Am. Chem. Soc. 131(24), 8362-8363 (2009)
223. "Unambiguous Determination of Isobaric Histone Modifications by Reverse-Phase Retention Time and High-Mass Accuracy". Lanhao Yang, Shengjiang Tu, Chen Ren, Esther M. M. Bulloch, Chung-Lin Liao, Ming-Daw Tsai, Michael A. Freitas, Anal. Biochem. 396, 13-22 (2010).
224. "JNK-Mediated Turn-Over and Stabilization of the Transcription Factor p45/NF-E2 During Differentiation of Murine Erythroleukemia Cells". Tung-Liang Lee, Yu-Chiau Shyu, Pang-Hung Hsu, Chiung-Wen Chang, Shau-Ching Wen, Ming-Daw Tsai, and Che-Kun James Shen, Proc. Natl. Acad. Sci. 107, 52-57 (2010).
225. "Unique Catalytic Mechanism of Phosphatidylinositol-Specific Phospholipase C from Streptomyces antibioticus". Chaun Bai, Li Zhao, Ming-Daw Tsai, Karol S. Bruzik, J. Am. Chem. Soc., 132(4), 1210–1211 (2010).
226. "Catalytic Mechanism of DNA Polymerases". Michelle P. Roettger, Marina Bakhtina, Sandeep Kumar, and Ming-Daw Tsai, in “Comprehensive Natural Products II Chemistry and Biology”; Mander, L., Lui, H.-W, Eds.; Elsevier: Oxford; volume 8, pp349-383 (2010). (Review)
227. "Nonhydrolyzable Analogs of Phosphatidylinositol as Ligands of Phospholipases C." Cornelia Mihai , Xiangjun Yue, Li Zhao, Alex Kravchuk, Ming-Daw Tsai, and Karol S. Bruzik, New J. Chem. 34, 925-933 (2010). (Review)
228. "Global Analysis of Modifications of Human BK Virus Structural proteins by Mass Spectrometry", Fang, C.-Y.; Hsu, P.-H.; Chen, S.-Y.; Wang, M.; Chen, P.-L.; Chang, C.-F.; Chen, L.-S.; Shen, C.-H.; Ou, W.-C.; Juan, L.-J.; Tsai, M.-D.; Chang, D., Virology 402, 164-176 (2010).
229. "Contributions of conserved TPLH tetrapeptides to the conformational stability of ankyrin repeat proteins." Yi Guo, Chunhua Yuan, Feng Tian, Kun Huang, Christopher M. Weghorst, Ming-Daw Tsai, Junan Li, J. Mol. Biol. 399, 168-181 (2010).
230. "α-Helical burst on the folding pathway of FHA domains from Rad53 and Ki67”. Matsumura Y, Shinjo M, Mahajan A, Tsai MD, Kihara H, Biochimie 92, 1031-1039 (2010).
231. "Database Search Algorithm for Identification of Intact Cross-Links in Proteins and Peptides Using Tandem Mass Spectrometry”. Hua Xu, Pang-Hung Hsu, Liwen Zhang, Ming-Daw Tsai, and Michael A. Freitas, J. Proteomics Res. 9, 3384-3393 (2010).
232. "High-throughput identification of compounds targeting influenza RNA-dependent RNA polymerase activity". Ching-Yao Su , Ting-Jen R. Cheng, Meng-I Lin, Shi-Yun Wang, Wen-I Huang, Shao-Ying Lin-Chu, Yu-Hou Chen, Chung-Yi Wu, Michael M. C. Lai, Wei-Chieh Cheng, Ying-Ta Wu, Ming-Daw Tsai, Yih-Shyun E. Cheng, and Chi-Huey Wong, Proc. Natl. Acad. Sci. USA, 107, 19151-6 (2010).
233. "Regioselective deacetylation based on teicoplanin-complexed Orf2* crystal structures". Hsiu-Chien Chan, Yu-Ting Huang, Syue-Yi Lyu, Chuen-Jiuan Huang, Yi-Shan Li, Yu-Chen Liu, Chia-Cheng Chou, Ming-Daw Tsai, and Tsung-Lin Li, Molecular BioSystems, 7, 1224-1231 (2011).
234. "The histone H3K36 demethylase Rph1/KDM4 regulates the expression of the photoreactivation gene PHR1". Chung-Yi Liang, Pang-Hung Hsu, Dai-Fang Chou, Chao-Yu Pan, Wei-Chieh Huang, Ming-Daw Tsai, Wan-Sheng Lo, Nucl. Acids. Res. 39, 4151-4165 (2011).
235. "Interception of teicoplanin oxidation intermediates yields new antimicrobial scaffolds". Yu-Chen Liu, Yi-Shan Li, Syue-Yi Lyu, Li-Jen Hsu, Yu-Hou Chen, Yu-Ting Huang, Hsiu-Chien Chan, Chuen-Jiuan Huang, Gan-Hong Chen, Chia-Cheng Chou, Ming-Daw Tsai, and Tsung-Lin Li, Nature Chem. Biol. 7, 304-309 (2011).
236. "Functions of some capsular polysaccharide biosynthetic genes in Klebsiella pneumonia NTUH K-2044". Jin-Yuan Ho, Tzu-Lung Lin, Chun-Yen Li, Arwen Lee, An-Ning Cheng, Ming-Chuan Chen, Shih-Hsiung Wu, Jin-Town Wang, Tsung-Lin Li, and Ming-Daw Tsai, PLoS One, 6, e21664 (2011).
237. "Regulatory Mechanisms of Tumor Suppressor P16INK4A and Their Relevance to Cancer". Junan Li, Ming-Jye Poi, and Ming-Daw Tsai, Biochemistry 50, 5566-5582 (2011). (Review)
238. "The C-Terminus of Histone H2B Is Involved in Chromatin Compaction Specifically at Telomeres, Independently of Its Monoubiquitylation at Lysine 123". Chen-Yi Wang, Chia-Yin Hua, Hsiang-En Hsu, Chia-Ling Hsu, Hsin-Yi Tseng, Duncan E. Wright, Pang-Hung Hsu, Chih-Hung Jen, Chia-Yeh Lin, Meng-Ying Wu, Min-Daw Tsai, Cheng-Fu Kao, PLoS One, 6, e22209 (2011).
239. "Protein kinase A-mediated serine 35 phosphorylation dissociates histone H1.4 from mitotic chromosome". Chi-Shuen Chu, Pang-Hung Hsu, Pei-Wen Lo, Elisabeth Scheer, Laszlo Tora, Heng-Jen Tsai, Ming-Daw Tsai, and Li-Jung Juan, J. Biol. Chem. 286, 35843-35851 (2011)
240. "Kinetic mechanism of active site assembly and chemical catalysis of DNA Polymerase β". Paul B. Balbo, Eric Chun-Wei Wang, and Ming-Daw Tsai, Biochemistry 50, 9865-9875 (2011).
241. "E339…R416 salt bridge of nucleoprotein as a feasible target for influenza virus inhibitors". Yu-Fang Shen, Yu-Hou Chen, Shao-Ying Chu, Meng-I Lin, Hua-Ting Hsu, Pei-Yu Wu, Chao-Jung Wu, Hui-Wen Liu, Fu-Yang Lin, Gialih Lin, Pang-Hung Hsu, An-Suei Yang, Yih-Shyun E. Cheng, Ying-Ta Wu, Chi-Huey Wong, and Ming-Daw Tsai, Proc. Natl. Acad. Sci. USA, 108, 16515-16520 (2011).
242. "Phosphorylation of NuSAP by Cdk1 regulates its interaction with microtubules in mitosis". Han-Yi Chou, Ting-Huang Wang, Sheng-Chung Lee, Pang-Hung Hsu, Ming-Daw Tsai, Chih-Ning Chang, and Yung-Ming Jeng, Cell Cycle 10, 4083-4089 (2011).
243. "Structural delineation of MDC1 FHA domain binding with CHK2 pThr68". Hsin-Hui Wu, Pei-Yu Wu, Kai-Fa Huang, Yu-Ya Kao, and Ming-Daw Tsai, Biochemistry 51, 575-577 (2012).
244. "SUMOylation of Blimp-1 is critical for plasma cell differentiation". Ying, Hsia-Yuan; Su, Shin-Tang; Hsu, Pang-Hung; Chang, Che-Chang; Lin, I-Ying; Tseng, Yu-Hsuan; Chen, Mei-Yu; Tsai, Ming-Daw; Shih, Hsiu-Ming; Lin, Kuo-I, EMBO reports 13(7), 631-637 (2012).
245. "Intermolecular binding between TIFA-FHA and TIFA-pT mediates TNFα stimulation and NF-κB activation". Chia-Chi Flora Huang, Jui-Hung Weng, Tong-You Wade Wei, Pei-Yu Gabriel Wu, Pang-Hung Hsu, Yu-Hou Chen, Shun-Chang Wang, Dongyan Qin, Chin-Chun Hung, Shui-Tsong Chen, Andrew H.-J. Wang, John Y.-J. Shyy, and Ming-Daw Tsai, Mol. Cell Biol. 32(14), 2664-2673 (2012).
246. "Loss of NPGPx, an oxidative stress sensor, compromises GRP78 chaperone activity and causes systemic oxidative stress, high tumor incidence and shortened lifespan". Pei-Chi Wei, Yi-Hsuan Hsieh, Mei-I Su, X.-J. Jiang, Pang-Hung Hsu, Wen-Ting Lo, Yi-Cheng Chang, Yung-Ming Jeng, Ju-Ming Wang, Phang-lang Chen, Kao-Fen Lee, Ming-Daw Tsai, Jin-Yuh Shew and Wen-Hwa Lee, Mol. Cell 48, 747-759 (2012).
247. "Amino acid substitutions of MagA in Klebsiella pneumoniae affect the biosynthesis of the capsular polysaccharide". Tzu-Lung Lin, Feng-Ling Yang, Tsung-Lin Li, An-Suei Yang, Ming-Daw Tsai, Shih-Hsiung Wu, Jin-Town Wang, PLoS One, 7, e46783 (2012).
248. "Ubc9 acetylation modulates distinct SUMO target modification and hypoxia response". Yung-Lin Hsieh, Hong-Yi Kuo, Mandar T. Naik, Che-Chang Chang, Pei-Hsin Liao, Chun-Chen Ho, Tien-Chi Huang, Jen-Chong Jeng, Pang-Hung Hsu, Ming-Daw Tsai, Tai-Huang Huang, and Hsiu-Ming Shih, EMBO J. 32, 791-804 (2013).
249. "Reversible acetylation regulates SIK2 and its function in autophagy". Fu-Chia Yang, Bertrand Tan, Wei-Hao Chen, Ya-Huei Lin, Jing-Yi Huang, Hui-Yu Sun, Pang-Hung Hsu, Gunn-Guang Liou, James Shen, Ching-Jin Chang, Chau-Chung Han, Ming-Daw Tsai, and Sheng-Chung Lee, J. Biol. Chem. 288, 6227-6237 (2013).
250. "Phosphorylation of mRNA Decapping Protein Dcp1a by the ERK Signaling Pathway during Early Differentiation of 3T3-L1 Preadipocytes". Chiang PY, Shen YF, Su YL, Kao CH, Lin NY, Hsu PH, Tsai MD, Wang SC, Chang GD, Lee SC, Chang CJ, PLoS One, 8, e61697 (2013).
251. "Histone Demethylase RBP2 Promotes Lung Tumorigenesis and Cancer Metastasis". Yu-Ching Teng, Cheng-Feng Lee, Ying-Shiuan Li, Yi-Ren Chen, Pei-Wen Hsiao, Meng-Yu Chan, Feng-Mao Lin, Hsien-Da Huang, Yen-Ting Chen, Yung-Ming Jeng, Chih-Hung Hsu, Qin Yan, Ming-Daw Tsai, and Li-Jung Juan, Cancer Research 73, 4711-4721 (2013).
252. "Evidence that P12, a specific variant of P16INK4A, plays a suppressive role in human pancreatic carcinogenesis". Poi MJ, Knobloch TJ, Yuan C, Tsai MD, Weghorst CM, Li J., Biochem. Biophys. Res. Commun. 436, 217-222 (2013).
253. "Molecular basis of the essential S phase function of the Rad53 checkpoint kinase". Hoch NC, Chen ES, Buckland R, Wang SC, Fazio A, Hammet A, Pellicioli A, Chabes A, Tsai MD, Heierhorst J, Mol Cell Biol. 33, 3202-3213 (2013).
254. "Interaction between Salt-inducible Kinase 2 (SIK2) and p97/VCP Regulates ER-associated Protein Degradation in Mammalian Cells". Yang FC, Lin YH, Chen WH, Huang JY, Chang HY, Su SH, Wang HT, Chiang CY, Hsu PH, Ming-Daw Tsai, Tan BC, Sheng-Chung Lee, J. Biol. Chem. 288, 33861-33872 (2013)..
255. "Use of quantitative mass spectrometric analysis to elucidate the mechanisms of phospho-priming and auto-activation of the checkpoint kinase Rad53 in vivo". Eric S.-W. Chen, Nicolas C. Hoch, Shun-Chang Wang, Achille Pellicioli, Jorg Heierhorst, and Ming-Daw Tsai, Mol Cell Proteomics 13, 551-565 (2014).
256. "Biosynthesis of Streptolidine Involved Two Unexpected Intermediates Produced by a Dihydroxylase and a Cyclase with Unusual Mechanisms". Chin-Yuan Chang, Syue-Yi Lyu, Yu-Chen Liu, Ning-Shian Hsu, Chih-Chung Wu, Cheng-Fong Tang, Kuan-Hung Lin, Jin-Yuan Ho, Chang-Jer Wu, Ming-Daw Tsai, & Tsung-Lin Li, Angewandte Chemie 53, 1943-1948 (2014).
257. "Tight Regulation of a Timed Nuclear Import Wave of EKLF by PKCq and FOE during Pro-E to Baso-E Transition". Yu-Chiau Shyu, Tung-Liang Lee, Xin Chen, Pang-Hung Hsu, Shau-Ching Wen, Yi-Wei Liaw, Chi-Huan Lu, Po-Yen Hsu, Mu-Jie Lu, JauLang Hwang, Ming-Daw Tsai, Ming-Jing Hwang, Jim-Ray Chen, and Che-Kun James Shen, Dev Cell. 28, 409-422 (2014).
258. "Fha Interaction with Phosphothreonine of TssL Activates Type VI Secretion in Agrobacterium tumefaciens". Jer-Sheng Lin, Hsin-Hui Wu, Pang-Hung Hsu, Lay-Sun Ma, Yin-Yuin Pang, Ming-Daw Tsai, and Erh-Min Lai, PLOS Pathogens, 10(3), e1003991 (2014).
259. "Structure and mechanism of a non-heme iron-SAM dependent C-methyltransferase and its engineering to hydratase and O-methyltransferase". Xiao-Wei Zou, Yu-Chen Liu, Ning-Shian Hsu, Chuen-Jiuan Huang, Syue-Yi Lyu, Hsiu-Chien Chan, Chin-Yuan Chang, Hsien-Wei Yeh, Kuan-Hung Lin, Chang-Jer Wu, Ming-Daw Tsai and Tsung-Lin Li, Acta Crystallographica Section D, 70, 1549-1560 (2014).
260. "How a low-fidelity DNA polymerase chooses non-Watson-Crick from Watson-Crick incorporation". Wen-Jin Wu, Mei-I Su, Jian-Li Wu, Sandeep Kumar, Liang-hin Lim, Chun-Wei Eric Wang, Frank H. T. Nelissen, Ming-Chuan Chad Chen, Jurgen F. Doreleijers, Sybren S. Wijmenga, and Ming-Daw Tsai, J. Am. Chem. Soc. 136, 4927-4937 (2014). [Featured in ASBMB TODAY http://wildtypes.asbmb.org/2014/03/20/how-a-polymerase-bypasses-the-rules-of-watson-crick-base-pairing/ ] [ http://www.asbmb.org/asbmbtoday/ ]
261. "How DNA Polymerases Catalyze DNA Replication, Repair, and Mutation". Ming-Daw Tsai, Biochemistry, 53(17), 2749-2751 (2014).
262. "Multiple complexes of long-aliphatic N-acyltransferases led to synthesis of 2,6-diacylated/2-acyl-substituted glycopeptide antibiotics effectively killing VRE". Lyu, Syue-Yi; Liu, Yu-Chen; Chang, Chin-Yuan; Huang, Chuen-Jiuan; Chiu, Ya-Huang; Huang, Chun-Man; Hsu, Ning-Shian; Lin, Kuan-Hung; Wu, Chang-Jer; Tsai, Ming-Daw; Li, Tsung-Lin, J. Am. Chem. Soc. 136, 10989-10995 (2014).
263. "Ultrafast water dynamics at the interface of polymerase-DNA binding complex". Yi Yang, Yangzhong Qin, Qing Ding, Marina Bakhtina, Lijuan Wang, Ming-Daw Tsai, and Dongping Zhong, Biochemistry 53, 5405-5413 (2014).
264. "PP2A and Aurora differentially modify Cdc13 to promote telomerase release from telomeres at G2/M phase". Zih-Jie Shen , Pang-Hung Hsu , Yu-Tai Su , Chia-Wei Yang , Li Kao , Shun-Fu Tseng , Ming-Daw Tsai, and Shu-Chun Teng, Nature Commun. 5:5312. doi: 10.1038/ncomms6312 (2014).
265. "RDE-8 encodes an endoribonuclease required for RNA interference in C. elegans". Hsin-Yue Tsai, Chun-Chieh G. Chen, James Moresco, Darryl Conte Jr, Daniel A. Chaves, Shohei Mitani, John Yates III, Ming-Daw Tsai, and Craig C. Mello, Cell accepted.
266. "NIFK overexpression is associated with poor survival of lung cancer and promotes tumor invasion through casein kinase 1α". Tsung-Chieh Lin, Chia-Yi Su, Tsung-Ching Lai, Yi-Hua Jan, Chi-Tai Yeh, Chi-Long Chen, Luo-Ping Ger, Hong-Tai Chang, Chih-Jen Yang, Ming-Shyan Huang, John Y.-J. Shyy, Ming-Daw Tsai, and Michael Hsiao, submitted.
267. "NIFK regulates cell cycle progression through pre-rRNA processing". Wen An Pan, Hsin-Yue Tsai, Pei-Yu Gabriel Wu, and Ming-Daw Tsai, submitted.
268. "PHRF1 links methylated H3K36 and NBS1 to non-homologous end joining". Chi-Fang Chang, Ming-Ying Yu, Jin-Yu Lee, Po-Chen Chu, Pei-Yu Wu, and Ming-Daw Tsai, and Mau-Sun Chang, Cell Death and Disease, in revision.

Invited Lectures

1. "31P(17O) NMR Studies of Metal-Nucleotide Interactions ". Gordon Conference on Enzymes, Coenzymes, and Metabolic Pathways, July 7-11, 1980.
2. "Biochemical Application of NMR Methods Involving Oxygen Isotopes". Purdue + University Biochemistry Program, January 18, 1982.
3. "Biochemical Application of 17O NMR and 31P(17O) NMR". Federation Meeting, Minisymposium on Spectroscopic Applications of 17O in Biological Chemistry, April 20, 1982.
4. "Phospholipids Chiral at Phosphorus, Stereochemistry of Reactions Catalyzed by Phospholipases". University of Chicago, Biophysics and Theoretical Biology, March 11, 1983.
5. "NMR, Chirality, Enzymes, and Membranes". Gordon Conference on Enzymes, Coenzymes, and Metabolic Pathways," July 4, 1983.
6. "Phospholipids Chiral at Phosphorus. Use of Chiral Thiophosphatidylcholine to Study the Metal-Binding Properties of Bee Venom Phospholipase A2". Fourth Midwest Enzyme Conference, Chicago, IL, October 27, 1984.
7. "Synthesis and Biochemical Properties of Phospholipids Chiral at Phosphorus" . Brown University, Dept. of Chemistry, March 1, 1985.
8. "17O NMR Study on the Interaction of Adenine Nucleotides with Metal Ions and with Myokinase". ACS 189th National Meeting, Symposium on Biological NMR, Miami Beach, May 1, 1985.
9. "Phospholipids Chiral at Phosphorus. Use of Chiral Thiophospholipids to Study the Mechanism of Phospholipase A2". Steenbock Symposium on Stereochemistry of Enzymatic Reactions, University of Wisconsin, Madison, July 1-3, 1985.
10. "Use of 17O NMR and 31P NMR to Study Metal-Nucleotide-Enzyme Interactions". University of Maryland, Dept. of Chemistry, November 5, 1985
11. "Phospholipids Chiral at Phosphorus". Gordon Conference on "Enzymes, Coenzymes, and Metabolic Pathways", June 29 - July 4, 1986
12. "Phospholipids Chiral at Phosphorus. Stereochemistry of Enzymatic Reactions" Tenth International Conference on Phosphorus Chemistry, Bonn, W. Germany, August 31 - September 6, 1986.
13. "Phospholipids Chiral at Phosphorus". Second International Symposium on Phosphorus Chemistry Directed Toward Biology, Poland, September 7-12, 1986.
14. "Phosphorothioates: Preparation and Utilization of Phosphate Analogs". University of Texas, Health Science Center at San Antonio, Department of Biochemistry, Feb. 26, 1987.
15. "Biochemical and Biophysical Properties of Phospholipids Chiral at Phosphorus". University of Texas, Health Science Center at San Antonio, epartment of Biochemistry, February 27, 1987.
16. "Phospholipids Chiral at Phosphorus". University of Kentucky, Department of Chemistry, March 13, 1987.
17. "Phospholipids Chiral at Phosphorus". University of Illinois, College of Medicine at Chicago, Department of Biological Chemistry, May 7, 1987.
18. "Phospholipids Chiral at Phosphorus". ACS 21st Middle Atlantic Regional Meeting, Pomona, NJ, May 20, 1987.
19. "Phospholipids Chiral at Phosphorus". University of Akron, Department of Chemistry, September 15, 1987.
20. "Gene Synthesis and Protein Engineering". OSU Industrial/Academic Chemistry Program, October 10, 1987.
21. "Is There Interfacial Activation in the Catalysis of Phospholipase A2? " Seventh Midwest Enzyme Conference, University of Chicago, October 17, 1987.
22. "Phospholipids Chiral at Phosphorus". Second SCBA International Symposium and Workshop, Symposium on Bioorganic and National Product Chemistry,Berkeley, June 27-30, 1988.
23. "Stereochemistry of Phospholipases". Smith and Kline and French Laboratories, November 17, 1988.
24. "Use of Site-Directed Mutagenesis to Study the Structure-Function Relationship of Adenylate Kinase". Ohio State Biochemistry Program, February 21, 1989.
25. "Bioorganic Chemistry: From Small Molecules to Macromolecules". University of Washington, Seattle, Department of Chemistry, March 10, 1989.
26. "Stereochemistry and Mechanism of Phospholipases". Boston University, Department of Chemistry, March 20, 1989.
27. "Enzyme Mechanisms: From Substrate Engineering to Protein Engineering". Dept. of Biochemistry and Molecular Biology, Univ. of Chicago, May 10, 1989.
28. "Mechanism of Adenylate Kinase". Dept. of Biophysics, Max-Planck Institute for Medical Research, Heidelberg, West Germany, June 14, 1989.
29. "Mechanism of Adenylate Kinase". Institute of Organic and Biochemistry, University of Freiburg, Freiburg, West Germany, June 15, 1989.
30. "Bioorganic Chemistry: From Conventional to Contemporary". Institute of Chemistry, Academia Sinica, Taipei, Taiwan, Sept 4, 1989.
31. "Bioorganic Chemistry: From Conventional to Contemporary". Institute of Enzyme Research, University of Wisconsin at Madison, Sept. 14, 1989.
32. "Mechanism of Adenylate Kinase". Department of Biochemistry and Biophysics, Texas A&M University, November 1, 1989.
33. "Structural and Functional Studies on the Mechanism of Adenylate Kinase". Department of Chemistry, New Mexico State University, March 8, 1990.
34. "Gene Synthesis, Expression, and Protein Engineering of Phospholipase A2 from Bovine Pancreas". Department of Biochemistry and Molecular Biology, New Mexico State University, March 9, 1990.
35. "Perfecting an Enzyme: A phospholipase A2 with Significantly Improved Catalytic Activity". American Chemical Society National Meeting, Boston, Apr. 22-27, 1990.
36. "Enzyme Mechanisms: From Substrate Engineering to Protein Engineering". Third SCBA International Symposium and Workshop, Hong Kong, June 26-30, 1990.
37. "Protein Engineering of Phospholipase A2 From Bovine Pancreas". Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan, July 2, 1990.
38. "A Chemist's Approach to Biomedical Research". Veterans General Hospital, Taipei, Taiwan, July 3, 1990.
39. "New Approaches in Bioorganic Chemistry". Department of Chemistry, National Taiwan University, Taipei, July 9, 1990.
40. "Structure-Function Studies of Large Organic Molecules-Enzymes". International Symposium for Chinese Organic Chemists, Shanghai, July 12-14, 1990.
41. "Mechanism of Adenylate Kinase: Have Previous NMR and X-Ray Results Passed the Test by Site-Directed Mutagenesis?" Monsanto Company, St. Louis, August 7, 1990.
42. "Modern Bioorganic Chemistry: Structure-Function Studies of Enzymes". Department of Chemistry, St. Olaf College, Northfield, Minnesota, Setp. 14, 1990.
43. "Mechanism of Adenylate Kinase: Site-Directed Mutagenesis Versus X-Ray and NMR ", Kent State University, Kent, Ohio, October 4, 1990.
44. "Mechanism of Adenylate Kinase: Have Previous NMR and X-Ray Results Passed the Test by Site-Directed Mutagenesis?" Department of Chemistry, University of Delaware, November 5, 1990.
45. "Mechanism of Adenylate Kinase: Site-Directed Mutagenesis Versus X-Ray and NMR ". Department of Chemistry, Washington State University, Pullman, WA, Feb. 19, 1991.
46. "Protein Engineering of Phospholipase A2". Department of Chemistry, California Institute of Technology, Pasadena, CA, Feb. 27, 1991. (Joint Organic Chem./Chem. Biology)
47. "Protein Engineering of Phospholipase A2". School of Pharmacy, University of Cincinnati, Cincinnati, Ohio, April 4, 1991.
48. "Protein Engineering of Bovine Pancreatic Phospholipase A2". Royal Netherlands Academy of Arts and Sciences, Colloquium on Molecular Biology and Inhibition of Cellular and Extracellular Phospholipases A2, Amsterdam, April 23-27, 1991.
49. "Mechanism of Adenylate Kinase: Manipulating Phosphorus Stereochemistry by Site-Directed Mutagenesis". Regional Meeting of the American Chemical Society, Indianapolis, May 29-31, 1991.
50. "Phospholipase A2 Engineering". National Meeting of the American Chemical Society, New York City, Aug. 25-30, 1991.
51. "Structure-Function Relationship of Adenylate Kinase", Fox Chase Institute for Cancer Research, Philadelphia, September 19, 1991.
52. "Manipulating the Phosphorus Stereospecificity of an Enzyme", Dept. of Chemistry, University of Chicago, Nov. 15, 1991.
53. "Iterative Structure-Function Studies of Enzymes: A Case in Adenylate Kinase", Institute of Biological Chemistry, Academia Sinica, Taipei, Dec. 27, 1991.
54. "Modern Bioorganic Chemistry: Structure-Function Relationship of Enzymes", Institute of Chemistry, Academia Sinica, Taipei, Dec. 30, 1991.
55. "Structure-Function Relationship of Adenylate Kinase", Dept. of Biology, Syracuse University, March 6, 1992. (host: Richard Levy)
56. "Structure-Function Relationship of Adenylate Kinase", University of Florida College of Medicine, March 26, 1992. (host: David Silverman)
57. "Biochemical Applications on NMR", Dept. of Chemistry, Denison University, Granville, Ohio, April 23, 1992. (host: Richard Doyle)
58. "Structure-Function Relationship of Adenylate Kinase", Wright State University, Dayton Ohio, April 24, 1992. (host: Lawrence Prochaska)
59. "Stereochemical Mechanism of Phospholipase C", FASEB Summer Conference on Phospholipases, Saxtons River, Vermont, July 12-17, 1992. (Organizer: Ed Dennis)
60. "Structure-Function Relationship of Adenylate Kinase", Biotechnology Research Institute, National Research Council of Canada, Montreal, Sept. 2, 1992. (Host: Feng Ni)
61. "Structure-Function Relationship of Adenylate Kinase", Max-Planck Institute, Gottingen, Germany, Setp. 14, 1992. (Host: Fritz Eckstein)
62. "Structure-Function Relationship of Adenylate Kinase", EMBO Workshop, Germany, Sept. 16-19, 1992. (Organizer: Fred Wittinghofer)
63. "Structure-Function Relationship of Phospholipase A2", Dept. of Chemistry, Indiana University, Oct. 23, 1992. (Host: David Daleke)
64. "Structure-Function Relationship Enzymes: a Case Study with Phospholipase A2 ", Park Davis, Ann Arbor, May 4, 1993. (Host: Don Hupe)
65. "Structure-Function Relationship of Adenylate Kinase", Ann Arbor Enzymes Club, May 5, 1993. (Host: James Coward)
66. "Modern Bioorganic Chemistry: Structure-Function Relationship of Enzymes", Department of Chemistry, Tsing-Hua University, Taiwan, July 12, 1993.
67. "Improving the Structure-Function Relationship of Enzymes", Institute of Chemistry, Academia Sinica, Taipei, July 13, 1993.
68. "Structure-Function Relationship of Phospholipase A2", Dept. of Chemistry, Wayne State University, Sept. 24, 1993. (Host: Shahriar Mobashery)
69. "Mechanism of Adenylate Kinase. 1H, 13C, and 15N NMR Assignments, Secondary Structures, and Substrate Binding Sites", Thirteenth Midwest Enzyme Chemistry Conference, Chicago, October 9, 1993.
70. "NMR Analysis of the Structure of the Adenylate Kinase-MgAP5A Complex ", International Symposium on Adenylate Kinase, Yamaguchi University, Japan, March 26-28, 1994.
71. "Adenylate Kinase: From Molecular Biology to NMR Structure", Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, March 28, 1994. (Host: Bai-Ling Lin)
72. "Structure-Function Relationship of Adenylate Kinase", Chicago Medical School, Chicago, May 5, 1994. (Host: Bob Kemp)
73. "Assignment and Secondary Structures of a 22 kDa System at pH 7.1: Adenylate Kinase Complex with MgAP5A", Varian NMR Users Conference, Akron, August 18, 1994. (Host: Peter Rinaldi)
74. "Structure-Function Relationship of Adenylate Kinase", Floss Symposium, Seattle, Washington, August 27, 1994.
75. "Structure-Function Relationship of Adenylate Kinase", School of Pharmaceutical Sciences, Univ. of California at San Francisco, San Francisco, Sept. 15, 1994. (Host: George Kenyon)
76. "Structure-Function Relationship of Phospholipase A2", Department of Medicinal Chemistry, University of Illinois at Chicago, October 3, 1994. (Host: Karol S. Bruzik)
77. "Adenylate Kinase: From Molecular Biology to Total Assignment by NMR", Department of Chemistry, University of Illinois at Chicago, October 4, 1994. (Host: Wan-Hua Cho)
78. "Structure-Function Relationship of Adenylate Kinase", Dept. of Biophysics and Physiology, Case Western Reserve University, October 17, 1994. (Host: C. R. Sanders)
79. "Syntheses and Biochemical Applications of Phosphoinositides", The 4th Tohwa University International Symposium on Chemistry on the Biologically and Physiologically Active Natural Products, Fukuoka, Japan, November 19-22, 1994.
80. "Modern Bioorganic Chemistry: Structure-Function Relationship of Enzymes." Department of Chemistry, Case Western Reserve University, Cleveland, March 9, 1995. (Host: Michael Zagorski)
81. "Structure-Function Relationship of Adenylate Kinase." Department of Chemistry, Miami University, Oxford, Ohio, March 30, 1995. (Host: John F. Sebastian)
82. "Syntheses and Biochemical Applications of Phosphoinositides", Institute of Chemistry, Academia Sinica, Taipei, May 6, 1995.
83. "Structural Analysis of a 22 kDa System at pH 7.1 by NMR: Adenylate Kinase Complex with MgAP5A", Midwest Regional ACS Meeting, Akron, May 31, 1995
84. "Structural Determination of Adenylate Kinase, A 22 kD Protein", Fourteenth American Peptide Symposium, Columbus, June 18-23, 1995.
85. "From Protein Engineering to Drug Design". International SCBA Symposium on Rational Drug Design, Vancouver, June 25-30, 1995.
86. "Adenylate Kinase: A Model or an Exception?", Gordon Conference on Enzymes, Coenzymes, and Metabolic Pathways, New Hampshire, July 16-21, 1995.
87. "Structure-Function Relationship of Phospholipase A2", FASEB Summer Conference on Phospholipases, Vermont, July 22-27, 1995.
88. "Protein Structural Analysis by NMR", Department of Physics, Indiana University-Purdue University at Indianapolis, December 7, 1995.
89. "Design and Construction of a New Restriction Endonuclease Specific to the HIV Genome", International Symposium on Perspectives on Protein Engineering, Le Corum Montpellier, France, March 2-6, 1996.
90. "Structure-Function Relationship of Adenylate Kinase", Department of Chemistry, SUNY Stony Brook, March 25, 1996. (Host: Nicole Sampson)
91. "Modern Bioorganic Chemistry", The Fourth International Symposium for Chinese Organic Chemists, Hong Kong, April 5-8, 1996.
92. "Structure-Function Relationship of DNA Polymerase β" EMBO Meeting on Nucleotidyl and Phosphoryl Transfer in the Protein and RNA World, Xanten, Germany, Sept. 29 - Oct. 3, 1996.
93. "Structure-Function Relationship of Tumor Suppressor P16." Cancer Center, Ohio State University, November 6, 1996. (Host: Lee Johnson)
94. "Protein Engineering". Department of Chemical Engineering, Ohio State University, December 5, 1996. (Host: Shang-Tien Yang)
95. "Structural Analysis of Tumor Suppressor P16 by NMR". American Chemical Society Regional Meeting, Midland, Michigan, May 27, 1997.
96. "Adenylate Kinase: Site-directed Mutagenesis Versus NMR and X-ray". Protein Engineering Group, University of Toronto, May 29, 1997. (Host: Robert Reedjik)
97. "Probing Enzyme Mechanisms with Bridging and Nonbridging Sulfur Analogs of Nucleotides and Phospholipids". Department of Chemistry, University of Toronto, May 30, 1997. (Host: Andrew Woolley)
98. "Structure-Function Relationship of Bacterial PI-Phospholipase C." National Meeting of the American Chemical Society, Las Vegas, Sept. 7-11, 1997.
99. "Mechanism of PI-Specific Phospholipase C". Dept of Chemistry, Scripps Research Institute, September 12, 1997. (Host: Chi-Huey Wong)
100. "Structure-Function Relationship of PI-specific Phospholipase C". Dept of Biochemistry, Michigan State University, November 3, 1997. (Host: Honggao Yan).
101. "Mechanism of PI-Specific Phospholipase C". Dept of Pharmacology, Albert Einstein School of Medicine, New York, December 8, 1997. (Host: Zong-Yin Zhang)
102. "Structure-Function Relationship of Adenylate Kinase". University of Texas at Austin, January 23, 1998. (Host: Jon Robertus)
103. "Solution Structure of Tumor Suppressor p16 by NMR". Otterbein University, Westerville, Ohio, April 15, 1998. (Host: Chihae Yang)
104. "Structure and Function of Tumor Suppressor p16". Dept. of Chemistry, Florida State University, April 28, 1998. (Host: Alan Marshall)
105. Structure and Mechanism of Tumor Suppressor p16INK4A”. Department of Biochemistry, Case Western Reserve University, May 6, 1998.
106. "Use of Thiophosphate Analogs to Probe the Mechanism of PI-Phospholipase C". 14th International Conference on Phosphorus Chemistry, Cincinnati, July 12-17, 1998.
107. "Structure-Function Analysis of the INK4 Family of Tumor Suppressors". Department of Biochemistry, University of Toledo, October 15, 1998. (Host: James Slama)
108. "A Novel, Dual-Function Catalytic Triad Arg-Asp-His for P-O Bond Cleavage Catalyzed by PI-PLC". ASBMB Fall Symposia, Lake Tahoe, CA, Oct 23-26, 1998.
109. "Structure-Function Analysis of the INK4 Family of Tumor Suppressors", NMR Technologies: Development and Applications Conference, Baltimore, October 29-30, 1998.
110. "A Novel, Dual-Function Catalytic Triad Arg-Asp-His for P-O Bond Cleavage Catalyzed by PI-PLC". Dept of Chemistry, National Taiwan University, Nov. 10, 1998.
111. "Structure-Function Analysis of the INK4 Family of Tumor Suppressors". Dept of Chemistry, Tsinghua University, Nov. 11, 1998.
112. "A Novel, Dual-Function Catalytic Triad Arg-Asp-His for P-O Bond Cleavage Catalyzed by PI-PLC". Institute of Chemistry, Academia Sinica, Nov. 13, 1998.
113. "Structure-Function Analysis of the INK4 Family of Tumor Suppressors". Department of Chemistry, Kent State University, November 19, 1998. (Host: Scott Prosser)
114. "Identification of a Novel Catalytic Triad Arg-Asp-His from PI-specific Phospholipase C". Canadian Society of Chemistry National Meeting, May 29-31, 1999.
115. "Solution Structure of the FHA2 Domain of RAD53". 31st Central Regional Meeting of the American Chemical Society, Columbus, Ohio, June 21-23, 1999.
116. "Solution Structures and Functional Analyses of Tumor Suppressors p16 and p18 ". SCBA International Symposium, Hong Kong, August 14-19, 1999.
117. "Structure and Function of a New Phosphoprotein Binding Domain FHA2 from Yeast Rad53." Midwest Enzyme Chemistry Conference, Chicago, October 2, 1999.
118. "New Structural Motifs for Protein-Protein Interactions in Cancer-related Pathways." University of Akron, November 23, 1999. (Host: Matthew Espe)
119. "Structure, Function, and Specificity of FHA, a New Phosphoprotein Binding Domain ". Keynote Speaker, Volcano Conference, Seattle, Feb. 25-27, 2000.
120. "Mechanism of PI-Specific Phospholipase C". FASEB Summer Conference on Phospholipases, Snowmass Village, Colorado, July 8-13, 2000.
121. "Structure, Function, and Specificity of FHA, a New Phosphoprotein Binding Domain ". Bruker Users Conference, Columbus, Ohio, October 5-6, 2000.
122. "Structure and Specificity of FHA, a New Phosphoprotein Binding Domain in Signal Transductions". OSBP Symposium Keynote Speaker, October 7, 2000.
123. "Structure, Function, and Specificity of FHA, a New Phosphoprotein Binding Domain with Dual Specificity." Cambridge Healthtech Institute Conference on Protein Structure. McLean, Virginia, October 26-27, 2000.
124. "Structure and Specificity of FHA, a New Phosphoprotein Binding Domain in Signal Transduction." Institute of Biophysics, Academia Sinica, Beijing, Oct. 31, 2000.
125. "Structure and Specificity of FHA, a New Phosphoprotein Binding Domain in Signal Transduction." Department of Chemistry, Beijing University, Nov. 3, 2000.
126. "Structure and Function of the INK4 Family of Tumor Suppressors." Institute of Biophysics, Academia Sinica, Beijing, Nov. 3, 2000.
127. Structure and Mechanism of Phospholipase A2 from Bovine Pancreas. Institute of Biophysics, Academia Sinica, Beijing, Nov. 7, 2000.
128. Structure and Specificity of FHA, a New Phosphoprotein Binding Domain in Signal Transduction. Department of Chemistry, Tsinghua University, Nov. 9, 2000.
129. Structure-Function Relationship of Phosphatidylinositol-specific Phospholipase C. Institute of Biophysics, Academia Sinica, Beijing, Nov. 10, 2000.
130. “Structure, Function, and Specificity of FHA, a New Phosphoprotein Binding Domain“. Eleventh International Conference on Second Messengers and Phosphoproteins, Melbourne, Australia, April 22-26, 2001.
131. “Structure, Function, and Specificity of FHA, a New Phosphoprotein Binding Domain“. Bioorganic Chemistry Gordon Conference, June 17-22, 2001.
132. "A DNA Polymerase with Specificity for Four Correct and One Mismatch Base Pairs ". Wayne State University Dept of Chemistry, Nov. 2, 2001. (Mark Spaller, host)
133. "Structure, Function, and Specificity of FHA, a New Phosphoprotein Binding Domain ". Department of Pharmacology, Ohio State University, Nov. 13, 2001. (Dale Hoyt, host)
134. "Chemistry at the Interface of Biology". Department of Chemistry, Central State University, Dayton, Ohio, Feb. 14, 2002. (Willie Houston, Host)
135. Symposium on "Modern Aspects of Structure Function Correlation of Biomolecules ", National Meeting of the American Chemical Society, Orlando, Florida, April 7-11, 2002.
136. "A Simplified Model for the Fidelity of DNA Polymerases". Gordon Research Conference on Mutagenesis, Main, July 28-Aug. 2, 2002.
137. "Structure and Mechanism of DNA Polymerases". UTMB Galvaston, TX, September 9, 2002. (James Lee, host)
138. "Chemical Basis of DNA Polymerase Fidelity – the Way We Survive". Evans Lecture Event, October 4, 2002.
139. "A Simplified Mechanism for the Fidelity of DNA Polymerases". Vanderbilt University, October 11, 2002.
140. "Structure and Mechanism of High-Fidelity and Low-Fidelity DNA Polymerases". Institute of Molecular Biology, Academia Sinica, October 21, 2002.
141. "A New View to the Fidelity Mechanism of High-Fidelity and Low-Fidelity DNA Polymerases". Michigan State University, Nov. 8, 2002. (Honggao Yan, host)
142. "Structure and Mechanism of High-Fidelity and Low-Fidelity DNA Polymerases". Texas A&M University, March 26, 2003. (Paul Fitzpatrick, host)
143. "Structure and Mechanism of High-Fidelity and Low-Fidelity DNA Polymerases". Thomas Jefferson University, Phidelphia, May 6, 2003. (Ya-ming Hou, host)
144. "Opportunities for Chemists in the Post-genomic Era". CACS-Tristate Chapter Symposium. Schering-Plough Research Institute, June 7, 2003.
145. "DNA Polymerases: from Chemistry to Biology", Dept of Chemistry, Tsinghua University, Beijing, Nov. 7, 2003
146. "DNA Polymerases: from Chemistry to Biology", Dept of Chemistry, Beijing University, Beijing, Nov. 10, 2003
147. "DNA Polymerases: from Chemistry to Biology", Dept of Chemistry, Tsinghua University, Hsinchu, Nov. 18, 2003
148. “Structure and Mechanism of High-Fidelity and Low-Fidelity DNA Polymerases“. Dept of Chemistry, Georgia State University, December 12, 2003. (Jenny Yang, host)
149. "Structure and Mechanism of High-Fidelity and Low-Fidelity DNA Polymerases". Dept of Biochemistry, Duke University, Jan 23, 2004. (Johannes Rudolph, host)
150. "Structural Basis of the Ligand Specificity of FHA Domains", Biophysics Program, Institute of Physics, Academia Sinica, Taipei, March 29, 2004. (Tsong Tian Yow, host)
151. "FHA Domains: from Chemistry to Biology", College of Life Sciences, Chiao-Tung University, Hsinchu, March 30, 2004. (Yuh-Shyong Yang, host)
152. "Enzymatic Evidence for a Low-Fidelity Base Excision Repair Pathway Encoded by African Swine Fever Virus", Research Division, Veterans Hospital, Taipei, April 2, 2004. (Ming-Shi Hsiao, host)
153. "Structural Basis of the Ligand Specificity of the FHA Domain of Human Tumor Suppressor Chk2“, Symposium on “Drug Discovery by Chemical Genomics Approach", 19th Joint Biological Societies Conference (JBSC), Taipei, April 10-11, 2004. (Also served as session chair.)
154. "NMR structures of proteins related to DNA damage and cancer". Structural Biology Group, NIH, May 10, 2004. (Host: Angela Gronenbon)
155. "Structure and Mechanism of a High-Fidelity and a Low-Fidelity DNA Polymerases", The 9th Symposium on Recent Advances in Biophysics, Taipei, May 26-28, 2004. (Keynote)
156. "NMR structures of proteins related to DNA damage and cancer". Symposium on structures, dynamics, and interactions of biological molecules, National Central University, June 4, 2004.
157. "NMR studies of proteins related to DNA damage and cancer". University of Carnegie Mellon, July 29, 2004 (Chien Ho, host).
158. "Stereochemistry Goes a Long Way in Phosphatidylinositol-specific Phospholipase C ", Frontiers of Bioorganic and Natural Product Chemistry Symposium, Seattle, August 26-29, 2004. (Also serve as chair of the organizing committee.)
159. "Structure and Function of FHA Domains in Signal Transduction". Tsinghua University, September 15, 2004.
160. "Learning the Transition State Structure of Phosphatidylinositol-specific Phospholipase C from Bioorganic Approaches", Eighth ISCOC International Symposium, Hong Kong, December 19-22, 2004.
161. "NMR studies of proteins related to DNA damage and cancer". Institute of Bioinformatics and Structural Biology, National Tsing Hua University, December 30, 2004. (Ping-Chiang Lyu, host)
162. "FHA Domains in Signal Transduction: from Chemistry to Biology", Department of Chemistry, Case Western Reserve University, Cleveland, Ohio, January 20, 2005. (Robert Salomon, host)
163. "Protein-Phosphoprotein Interactions – A New Frontier in Structural Biology. " National Defense University, Taipei, Taiwan, March 10, 2005.
164. "Chemical Approach to Biology: A Case for Caution". Symposium on Chemistry at the Interface of Biology, Salk Fork Resort, May 14, 2005.
165. "Structure, Function and Specificity of FHA Domains". Institute of Pasteur, May 23, 2005.
166. "Protein-Phosphoprotein Interactions – A New Frontier in Structural Biology. " Biophysical Society Meeting, Hsinchu, May 27, 2005. (Plenary lecture)
167. "When NMR Beats X-ray Crystallography in the Determination of Protein Structures. " Taiwan Magnetic Resonance Society, Taipei, May 28, 2005. (Inauguration lecture)
168. "Protein-Phosphoprotein Interactions – A New Frontier in Structural Biology. " National Taiwan University (Chemistry), Taipei, Taiwan, June 10, 2005.
169. "Protein-Phosphoprotein Interactions – A New Frontier in Structural Biology. " NHRI, Taiwan, Sept 02, 2005.
170. "When NMR Beats X-ray in Solving Protein Structures". First Asia-Pacific NMR Symposium, Japan, Nov. 10-11, 2005.
171. "FHA Domains in Signal Transduction: a Case for Caution in Chemical Biology" . Dept of Chemistry, SUNY at Buffalo, Nov. 30, 2005. (John Richard, host)
172. "Structural Biology of Cancer-relevant Proteins". NHRI Cancer Program, Taipei, June 9, 2006.
173. "Mechanism of action of high and low fidelity DNA polymerases". Gordon Research Conference on Enzymes, Coenzymes, and Metabolic Pathways, New Hampshire, July 16-21, 2006. (John Richard and Sue Miller, co-chairs)
174. "Identification of Histone Demethylases from Sacchromyces cerevisiae" . Biophysics Program, Ohio State University, October 4, 2006. (Ralph Bundschuh, host)
175. "Structural Biology of Cancer Proteins." Institutional Seminar of National Taiwan Univ. College of Medicine and University Hospital, December 27, 2006. (Host: 張美惠)
176. "Specificity of Phosphothreonine Recognition by FHA Domains". International Conference of Phosphorus Chemistry, Xiamen, China, April 15-21, 2007.
177. "Structure, Function and Specificity of Phosphothreonine-specific FHA Domains ". Dept of Chemistry, UC Davis, May 15, 2007. (Host: Xi Chen)
178. "FHA Domain Mediated Signaling Related to DNA Damage and Cancer". Cellular and Molecular Medicine Program, Academia Sinica, June 1, 2007.
179. "Specificity of Phosphothreonine Recognition by FHA Domains". XXIII International Conference on Yeast Genetics and Molecular Biology, Melbourne, Australia, July 2-6, 2007.
180. "FHA Domain - A Novel Phosphate Counting Switch for Sequential Activation of a Checkpoint Kinase Cascade", Eli Lilly, Indianapolis, October 4, 2007. (Host: Chuan Shih)
181. "A phospho-counting switch for sequential activation of a checkpoint kinase cascade". 2nd International Symposium on Bio-Inspired Engineering (ISBIE), October 8-10, 2007 at the Le Meridien Hotel, Dead Sea, Israel. (www.isbie.org)
182. "A phospho-counting switch for sequential activation of a checkpoint kinase cascade". The 2nd Asian-Pacific NMR Symposium, Oct. 12-14, 2007, Hsin-chu, Taiwan (Plenary Lecturer)
183. "Nuclear Protein NP as a Target for Inhibition of Viral Replication". International Symposium on Flu Virus, Oct 22, 2007, NHRI/NTUH, Taiwan.
184. "A phospho-counting switch for sequential activation of a checkpoint kinase cascade". International Symposium on Proteins: from Chemistry to Biology, October 24-26, 2007, Institute of Biological Chemistry, Academia Sinica, Taiwan.
185. "FHA Domain in Signal Transductions: from Chemistry to Biology". National Tsinghua University Dept of Chemistry, November 21, 2007.
186. "Oligomerization of the Nucleocapsid Protein as a Target for the Flu Virus". UC Davis – Academia Sinica Bilateral Conference, December 6-7, Taipei.
187. "FHA Domain in Signal Transductions: from Chemistry to Biology". Kaoshiung Medical Univdrsity. March 19, 2008.
188. "From the End to the Beginning". Great Lakes Regional ACS Meeting, Columbus, Ohio, June 14, 2008.
189. "A Phospho-counting Switch for Sequential Activation of a Checkpoint Kinase Cascade ". Toulouse University, France, July 7, 2008. (Host: Bernard Salles)
190. "Nucleocapsid Protein NP as a Target for Inhibition of Flu Virus Replication ". World Summit of Antivirals 2008 Kunming, China, July 20-22, 2008.
191. "FHA Domain in Signal Transduction: Chemistry, Structure, and Biology". Institute of Chemistry, Academia Sinica, Taipei, Sept 25, 2008. (Host: Sunney Chan).
192. "A Phospho-counting Switch for Sequential Activation of a Checkpoint Kinase Cascade in S. cerevisiae". Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Oct 1, 2008. (Host: Sunny Lo)
193. "FHA Domain in Signal Transduction: Chemistry, Structure, and Biology". National Cheng Kung University, Tainan, October 2, 2008.
194. "Structures of FHA domain complexes with phosphoprotein and phosphopeptides with single and multiple pThr sites". CSMRS-2, Hang-Chou, China, Oct 23-27, 2008.
195. "Structure, Function and Specificity of Phosphothreonine-specific FHA Domains ". Dept of Biochemistry, UC Riverside, Nov. 4, 2008. (Host: John Shyy)
196. "Structure, Function and Specificity of FHA Domains in DNA Damage Response Signaling". Department of Medicinal Chemistry, Purdue University, November 7, 2008.
197. "Structure and mechanism of a mutagenic DNA polymerase from African Swine Fever Virus". 4th International Symposium on Biocatalysis and Biotechnology, Academia Sinica, Taipei, Nov 19-21, 2008.
198. "Structure, Function and Specificity of Phosphothreonine-specific FHA Domains". National Yangming University, November 24, 2008. (Host: 魏耀揮)
199. "Cancer Research from the Angle of Structural Biology". “姆山生物醫學講座”, Taipei Medical University, Nov. 27, 2008.
200. "Counting Phosphates by FHA Domains". Taiwan – Japan Proteomics Symposium 2008. Academia Sinica, December 3, 2008.
201. "FHA, a signaling domain with diverse specificities". The 6th Asian Biophysical Association (ABA) Symposium, Hong Kong University of Science and Technology, Hong Kong, 11-14 January 2009.
202. "Molecular Interactions of Biophosphates – from Catalysis to Signaling". 24th Joint Annual Conference of Biomedical Sciences, Taipei, Taiwan, March 21-22, 2009. (Special Lecture)
203. "Protein-phosphoprotein interactions of phosphothreonine-specific FHA domains". Symposium of Nuclear Magnetic Resonance for Biological Macromolecules 2009 (SNBM2009), Hefei, China, June 25-29, 2009.
204. "Structure and Mechanism of Mutagenic DNA Polymerase X". 3rd Asian-Pacific NMR Conference, Korea, Oct 25-28, 2009.
205. "Protein-phosphoprotein interactions of phosphothreonine-specific FHA domains". Tsinghua University, Beijing, Nov. 24, 2009. (Host: Yigong Shi)
206. "Structure and Mechanism of Mutagenic DNA Polymerase X". Beijing Conference and Exhibition on Instrumental Analysis (BCEIA), Beijing, November 25-28, 2009. (Keynote speaker)
207. "Protein-phosphoprotein interactions of phosphothreonine-specific FHA domains". Nankai University, Tianjin, Nov. 27, 2009. (Host: George Wang)
208. "An Enzymologist's Approach to Histone Demethylases". The 5th Chromosome Biology Mini-Symposium. Institute of Molecular Biology, Academia Sinica, Taiwan, Dec. 2-3, 2009.
209. "A Phosphate-counting Mechanism for Sequential Activation of a Checkpoint Kinase Cascade". Department of Structural Biology, University of Pittsburgh. December 7, 2009.
210. "Structure and Mechanism of Mutagenic DNA Polymerase X". Taiwan Enzyme Mechanism Conference, Academia Sinica, Taipei, Taiwan, Dec 15, 2009.
211. "Protein-Protein Interactions as Potential Drug Targets". 7th International Symposium for Chinese Medicinal Chemists (ISCMC-2010), Kaoshiung Medical University, Feb. 1-5, 2010.
212. "Counting the Phosphates of a Phosphorylation Cluster". 2010 Disease Biomarker and TPS International Conference, Chang-Gung, Taipei, April 23-24, 2010.
213. "A Phosphate-counting Mechanism for Sequential Activation of a Checkpoint Kinase Cascade". ETH, Zurich, Switzerland, May 7, 2010.
214. "Mechanism of High-Fidelity and Mutagenic DNA Polymerases". International Conference on Evolving DNA Polymerases: Chemistry meets Biology, Monte Verita, Switzerland, May 9-14, 2010.
215. "Mechanism and specificity of Protein-phosphoprotein interactions". 10th Taiwan International Symposium on Statistical Physics: Critical Phenomena and Complex Biological Systems, Institute of Physics of Academia Sinica, July 27-31, 2010.
216. "A Phosphate-counting Mechanism for Sequential Activation of a Checkpoint Kinase Cascade". UCSD-Taiwan Symposium, San Diego, September 16-17, 2010.
217. "A Phosphate-counting Mechanism for Sequential Activation of a Checkpoint Kinase Cascade". National Institutes of Health, Washington DC, October 22, 2010.
218. "A Phosphate-counting Mechanism for Sequential Activation of a Checkpoint Kinase Cascade". Dept of Pharmacology, Johns Hopkins University, October 24, 2010.
219. "Protein-(phospho)protein interactions as potential drug targets". National Taiwan University College of Pharmacy, Nov. 8, 2010.
220. "蛋白質的奧密". National Chiao-Tung University, December 10, 2010.
221. "Structure and Mechanism of Mutagenic DNA Polymerase X". Pacifichem of American Chemical Society, Honolulu, December 16-20, 2010.
222. "Structural and Mechanistic Bases for the G:G Specificity of ASFV DNA Polymerase X". 22nd Enzyme Mechanisms Conference, Loews Don CeSar Beach Resort, St. Pete Beach, Florida, January 3-5, 2011.
223. "Structure and Mechanism of a Viral DNA Polymerase with G:G Specificity". Asian Biophysical Society Meeting, New Dehli, Jan 30 – Feb. 1, 2011.
224. "Structure and Mechanism of a Viral DNA Polymerase with G:G Specificity". 76th Israel Chemical Society Meeting, Tel Aviv, Feb 9-10, 2011.
225. "Structure and function of FHA domains in DNA damage and cancer signaling". National Chung-Hsing University, April 15, 2011.
226. "I nfluenza virus inhibitors that disrupt the nucleoprotein trimer". Academia Sinica – Johnson & Johnson Bilateral Symposium on Infectious Diseases, April 27-28, 2011.
227. "FHA, a signaling domain with diverse specificities and functions". FAOBMB Micro Symposium on "Advances in biochemistry and molecular biology for biomedical applications". Academia Sinica, Taipei, May 6, 2011.
228. "Structure and function of FHA domains in DNA damage and cancer signaling". 100年度國家衛生研究院生物醫學學術研討會, Chu-Nan, August 15-16, 2011.
229. "Influenza virus inhibitors that disrupt the nucleoprotein trimer". National Research Institute of Chinese Medicine, Taipei, Sept 21, 2011.
230. "FHA, a signaling domain with diverse specificities and functions". FAOBMB Conference, Singapore, October 5-7, 2011.
231. "How some error prone DNA polymerases overcome Watson-Crick base pairing". The 17th International Biophysics Congress (IUPAB), Beijing, Oct 31 – Nov. 3, 2011.
232. "Counting Phosphates". 2011 International Conference on Functional Proteomics: Advances in Post-translational Modification. Academia Sinica, Taipei, November 14-15, 2011.
233. "How some error prone DNA polymerases overcome Watson-Crick base pairing". 2nd Taiwan Enzyme Mechanism Conference, Taichung, December 15-16, 2011. (Plenary)
234. "A Phosphate-counting Mechanism for Sequential Activation of a Checkpoint Kinase". UC Davis, Sacramento, CA, Feb 17, 2012.
235. "Studies of proteins involved in cell cycle progression that are closely related with cancer and DNA damage". KMU Biomedical Symposium, March 21-22, 2012.
236. "Mechanism of Rad53 kinase activation in vivo - counting the phosphates by FHA domains". Department of Chemistry, UC Berkeley, Berkeley, CA, March 23, 2012.
237. "How many phosphorylation sites can a protein have, and what are the functions of multi-site phosphorylations?". Taiwan Proteomics Society meeting, May 31 – June 1, 2012, KMU.
238. "How a phosphorylation cluster regulates the activation and function of yeast checkpoint kinase Rad53". 8th International Symposium on Biocatalysis and Biotechnology, Sonoma Wine Country, CA, Oct 28-31, 2012.
239. "How a phosphorylation cluster regulates the activation and function of yeast checkpoint kinase Rad53". FAOBMB Congress, Bangkok, Thailand, November 25-29, 2012.
240. "Enzyme mechanisms from in vitro to in vivo". AS-CAS Bilateral Conference, Beijing, Dec 3-4, 2012.
241. "The entangled triangular relationship between structure, function, and mechanism of proteins". 21st Cell and Molecular Biology Frontier Science Symposium, Ken-Ding Park, 1/30 to 2/1, 2013. (Plenary Lecture)
242. "Protein-phosphoprotein complexes in DNA damage and cancer signaling". RIKEN-Academia Sinica Joint Conference, Taipei, March 2, 2013.
243. "The entangled triangular relationship between structure, function, and mechanism of proteins". National Central University, April 25, 2013.
244. "Functions of phosphorylation site clusters and multiple phosphorylations". 2013 Taiwan Proteomics Society International Conference: Recent Advances in Translational Medicine, Taipei Medical University, May 24-25, 2013.
245. "Protein-phosphoprotein complexes in DNA damage and cancer signaling". 12th HUPO World Congress, Yokohama, Japan, September 14-18, 2013.
246. "Protein-phosphoprotein complexes in DNA damage and cancer signaling". The 13th KIAS Conference on Protein Structure and Function, September 26-28, 2013.
247. "Nucleoprotein as a target for influenza virus inhibitors". Yonsei University, Seoul, Korea, September 30, 2013. (Host: Young-Ki Paik)
248. "How phospho-threonine recognition by FHA domains control diverse biological functions". University of Alberta, Alberta, Canada, October 25, 2013. (Host: Todd Lowary)
249. "How a low-fidelity DNA polymerase chooses non-Watson-Crick from Watson-Crick incorporation". 4th Conference of the Asia Pacific Protein Association (APPA2014), Jeju Island, Korea, May 17-20, 2014. [Plenary lecture]
250. "Chemical Specificity in Biology". 103年度生命科學組第29屆新科院士演講. Academia Sinica, June 27, 2014.
251. "Structural approach to tumor suppressors and cancer signaling". 2014 International Symposium for Recent Advances in Cancer (2014 ISRAC), Taipei Medical University, June 28, 2014.
252. "Mechanisms of phosphothreonine signaling and kinase activation". Department of Chemistry and Biochemistry, The Ohio State University, Columbus, Ohio, October 9, 2014.
253. "How a low-fidelity DNA polymerase chooses non-Watson-Crick from Watson-Crick incorporation". Korea-Taiwan Biophysical Conference, Daeyue, Korea, Nov 21-22, 2014.
254. "Mechanisms of phosphothreonine signaling and checkpoint kinase activation". 13th International Symposium for Chinese Organic Chemists (ISCOC-13), Xiamen, December 19-22, 2014. [Plenary Lecture]
255. "Mechanisms of phosphothreonine signaling and kinase activation". 24th Enzyme Mechanisms Conference, Galvaston, Texas, January 4-8, 2015.
256. "Unique mechanisms of phosphothreonine signaling in DNA damage response and cancer". 2015 Scripps meeting “Frontier in Biomedical Sciences”. Feb 14-16, 2015.
257. 生物醫學聯合學術年會, March 21, 2015 (Keynote Lecture)
258. 7th Beilstein ESCEC Symposium and 10th STRENDA meeting, in Rudesheim (Germany). September 14 - 18, 2015.
259. Pacifichem 2015, December 15-20, 2015.
Brief Summary of Research Accomplishment

Dr. Tsai’s research has focused on “Biophosphates: from Enzymes to Signaling”. He pioneered a widely adopted approach in which advanced bioorganic chemical methods are integrated with structural biological studies to uncover reaction mechanisms and signaling interactions involving biophosphates. Since biophosphates are central to biology and are involved in innumerable physiological functions, his research has had high impact that spans multiple disciplines of biological science, as highlighted below:
A. Chiral Phosphates, Thiophosphates and Mechanism of Adenylate Kinase. Tsai was among several major players who developed the field of chiral phosphates and thiophosphates. In 1979, he discovered the effect of 17O on 31P NMR, which made it possible to analyze the configuration of chiral [17O,18O] phosphates. He was also the first to develop synthesis and configurational analysis of chiral [16O,17O,18O,S] phosphate. He then used chiral dNTP analogs to resolve important mechanistic problems of muscle adenylate kinase, and to discover complete reversal and enhancement of phosphorus stereoselectivity by single mutations.
B. Phospholipids Chiral at Phosphorus and Mechanism of Phospholipases. Since 1982 Tsai pioneered syntheses and configurational analyses of phospholipids chiral at phosphorus and related phosphatidylinositol analogs, and used them to elucidate the mechanism of phospholipases A2, C, and D, which established his leadership in the field of phospholipase enzymology. Highlights include a 2001 “Chemical Reviews” article on phospholipase A2, and discovery of a novel catalytic triad Arg…Asp…His in phospholipase C.
C. Cyclin-dependent Kinase 4 (CDK4) Inhibitors – Ankyrin-repeat Tumor Suppressors. In 1998 Tsai reported the first structure of a new tumor suppressor p16, an inhibitor of CDK4, and subsequently determined other structures in the family (p15, p18, p19, and gankyrin). These and other studies led to a definitive review on ankyrin repeat proteins. This work contributed to the understanding of how the kinase activity of CDK4 is regulated by p16, work of fundamental relevance to both normal cellular proliferation and cancer.
D. Mechanism of DNA Polymerases. From 1998 Tsai used inert metal-dNTP complexes to resolve reaction intermediates of Pol , then characterized intermediate structures and measured microscopic rates. A major finding is that, in contrast to the then prevailing theory that fidelity is dictated by the rate-limiting, dNTP-induced conformational change, the latter is faster than the chemical step. In a related front, he discovered that Pol X from African swine fever virus is specific to G:G mispair in addition to Watson-Crick base pairs. He then solved the structures of Pol X and Pol X-DNA-MgdNTP ternary complex by NMR, and elucidated the structural and mechanistic basis for how Pol X overcomes Watson-Crick base pairing to achieve G:G mispair. He recently also demonstrated a new approach to design anti-influenza inhibitors by disrupting the trimeric structure of the nucleoprotein, leading to inhibition of the viral RNA polymerase and viral replication.
E. FHA Domain: Phosphothreonine Specificity and Phosphate Counting. Tsai is among the pioneers of structural and functional studies of the pThr-specific FHA domain. In 1999 Tsai reported the first structure of an FHA domain and subsequently established his leadership in this field with two important structures of protein-phosphoprotein complexes - human Ki67 FHA complexed with a phosphorylated domain of NIFK, and yeast Dun1 FHA complexed with the dual-phosphorylated SCD domain of Rad53. The latter led to discovery of a novel “phosphate counting mechanism” in signaling. In 2014 he further used quantitative MS analysis to elucidate the mechanisms of phospho-priming and auto-activation of Rad53 in vivo. Currently Tsai’s lab is actively investigating the biological functions and mechanisms of FHA domains from several human proteins in DNA damage response and cancer signaling, including CHK2, MDC1, and TIFA.
F. Histone Demethylases. In 2006, several labs discovered JmjC histone demethylases. In work extraneous to his studies of biophosphates, Tsai independently used global analyses of histones by mass spec to discover four histone demethylases in yeast. In addition, he determined the structure of the ARID domain of human histone demethylase RBP2 and identified its DNA binding specificity.